Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia.
Plasma metabolomics for the diagnosis and prognosis of H1N1 influenza pneumonia.
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DOI：
10.1186/s13054-017-1672-7
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发表时间：
2017-04-19
期刊：
影响因子：
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通讯作者：
Canadian Critical Care Translational Biology Group (CCCTBG)
中科院分区：
文献类型：
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作者：
Banoei MM;Vogel HJ;Weljie AM;Kumar A;Yende S;Angus DC;Winston BW;Canadian Critical Care Translational Biology Group (CCCTBG)
代谢组学是一种用于特定疾病的诊断和预后的工具。本研究的目的是检验代谢组学是否可以作为H1N1肺炎的潜在诊断和预后工具。我们的假设是，代谢组学可能用于早期诊断和预后的H1N1流感肺炎。 采用1H核磁共振波谱和气相色谱-质谱分析了42例H1N1肺炎患者、31例重症监护室（ICU）通气对照受试者和30例细菌性社区获得性肺炎患者在入院前24小时内采集的培养阳性血浆样本的代谢组，以进行疾病诊断和预后。我们发现，从入院后24小时内采集的样本中，基于血浆的代谢组学可用于区分H1N1肺炎和细菌性肺炎，以及H1N1肺炎的非幸存者和幸存者。此外，代谢组学是一种高度敏感和特异性的工具，用于H1N1肺炎死亡率的90天预后。这项研究表明，H1N1肺炎可以创建一个完全不同的血浆代谢谱从细菌培养阳性的肺炎和通气对照受试者在ICU的基础上，在24小时内的医院/ICU入院，在病程早期的血浆样本。本文的在线版本（doi：10.1186/s13054-017-1672-7）包含补充材料，可供授权用户使用。
Metabolomics is a tool that has been used for the diagnosis and prognosis of specific diseases. The purpose of this study was to examine if metabolomics could be used as a potential diagnostic and prognostic tool for H1N1 pneumonia. Our hypothesis was that metabolomics can potentially be used early for the diagnosis and prognosis of H1N1 influenza pneumonia. 1H nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry were used to profile the metabolome in 42 patients with H1N1 pneumonia, 31 ventilated control subjects in the intensive care unit (ICU), and 30 culture-positive plasma samples from patients with bacterial community-acquired pneumonia drawn within the first 24 h of hospital admission for diagnosis and prognosis of disease. We found that plasma-based metabolomics from samples taken within 24 h of hospital admission can be used to discriminate H1N1 pneumonia from bacterial pneumonia and nonsurvivors from survivors of H1N1 pneumonia. Moreover, metabolomics is a highly sensitive and specific tool for the 90-day prognosis of mortality in H1N1 pneumonia. This study demonstrates that H1N1 pneumonia can create a quite different plasma metabolic profile from bacterial culture-positive pneumonia and ventilated control subjects in the ICU on the basis of plasma samples taken within 24 h of hospital/ICU admission, early in the course of disease. The online version of this article (doi:10.1186/s13054-017-1672-7) contains supplementary material, which is available to authorized users.