Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities
Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2- and NO-induced antioxidant enzyme activities
复制标题
脱落酸可提高三倍体狗牙根的耐旱性，并涉及 H2O2 和 NO 诱导的抗氧化酶活性。
DOI：
10.1016/j.plaphy.2008.10.006
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发表时间：
2009-02-01
影响因子：
6.5
通讯作者：
Guo, Zhenfei
中科院分区：
文献类型：
--
作者：
Lu, Shaoyun;Su, Wei;Guo, Zhenfei
干旱是草坪草生长的主要限制因素。以三倍体狗牙根为材料，研究了脱落酸（阿坝）对干旱胁迫的保护作用及其与过氧化氢（H2O2）和一氧化氮（NO）的关系。阿坝处理显著提高了干旱胁迫下的相对含水量，降低了离子渗漏率和死株率。干旱胁迫下，ABA处理和对照植株的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性均有所增加，但ABA处理的活性更高。丙二醛，植物脂质过氧化反应的指标，ABA处理的植物比对照植物低，表明阿坝减轻干旱诱导的氧化损伤。阿坝处理增加了H_2O_2和NO含量。H_2O_2和NO清除剂及H_2O_2和NO生成抑制剂可阻断ABA诱导的SOD和CAT活性。结果表明，H_2O_2和NO是ABA诱导SOD和CAT活性所必需的。H_2O_2和NO均能单独诱导SOD和CAT活性。NO清除剂和NO生成抑制剂可阻断H2O2诱导的SOD和CAT活性，而H2O2清除剂和H2O2抑制剂则不能阻断NO诱导的SOD和CAT活性。结果表明，ABA诱导的SOD和CAT活性依次由H2O2和NO介导，NO作用于H2O2（C）2008 Elsevier Masson SAS下游。All rights reserved.
Drought is a major limiting factor for turfgrass growth. Protection of triploid bermudagrass against drought stress by abscisic acid (ABA) and its association with hydrogen peroxide (H2O2) and nitric oxide (NO) were investigated. ABA treatment increased relative water content, decreased ion leakage and the percentage of dead plants significantly under drought stress. Superoxide dismutase (SOD) and catalase (CAT) activities increased in both ABA-treated and control plants, but more in ABA-treated plants, under drought stress. Malondialdehyde, an indicator of plant lipid peroxidation, was lower in ABA-treated plants than in control plants, indicating that ABA alleviated drought-induced oxidative injury. ABA treatment increased H2O2 and NO contents. ABA-induced SOD and CAT activities could be blocked by scavengers of H2O2 and NO, and inhibitors of H2O2 and NO generation. The results indicated that H2O2 and NO were essential for ABA-induced SOD and CAT activities. Both H2O2 and NO could induce SOD and CAT activities individually. SOD and CAT induced by H2O2 could be blocked by scavenger of NO and inhibitors of NO generation, while SOD and CAT induced by NO could not be blocked by scavenger of H2O2 and inhibitor of H2O2. The results revealed that ABA-induced SOD and CAT activities were mediated sequentially by H2O2 and NO, and NO acted downstream of H2O2 (C) 2008 Elsevier Masson SAS. All rights reserved.