JNK signaling pathway is required for efficient wound healing in Drosophila
JNK signaling pathway is required for efficient wound healing in Drosophila
复制标题
DOI：
10.1006/dbio.2001.0502
复制
发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Manfruelli, P
中科院分区：
文献类型：
--
作者：
Rämet, M;Lanot, R;Manfruelli, P
有效的伤口愈合，包括凝血和随后的上皮再生是从昆虫到哺乳动物的动物从上皮损伤中恢复所必需的。这是可能的，涉及伤口愈合的基因是保守的，因此，果蝇可能是一个有用的体内模型系统，以确定在这一过程中所需的基因。此外，特定发育过程中的上皮运动（例如背侧闭合）与哺乳动物伤口愈合中观察到的运动相似。由于puckered（puc）基因是JUN N-末端激酶信号通路在背部闭合过程中的靶基因，我们研究了果蝇伤口愈合过程中puc基因的表达。我们发现，puc基因的表达是诱导的伤口边缘的上皮细胞和Jun激酶磷酸化受伤的表皮组织，这表明，JUN N-末端激酶信号通路被激活的表皮伤口产生的信号。在果蝇c-Fos同源物的情况下，puc基因的表达不再被诱导。最后，在JUN N-末端激酶缺陷的果蝇中受损的上皮修复表明，JUN N-末端激酶信号传导是在上皮伤口愈合开始时启动细胞形状变化所必需的。我们得出结论，胚胎JUN N-末端激酶基因盒在伤口边缘诱导。此外，果蝇似乎是一个很好的体内模型，以研究需要上皮再生的形态发生过程，如脊椎动物的伤口愈合。(C)2001年，爱思唯尔科学。
Efficient wound healing including clotting and subsequent reepithelization is essential for animals ranging from insects to mammals to recover from epithelial injury. It is likely that genes involved in wound healing are conserved through the phylogeny and therefore, Drosophila may be an useful in vivo model system to identify genes necessary during this process. Furthermore, epithelial movement during specific developmental processes, such as dorsal closure, ressembles of those seen in mammalian wound healing. As puckered (puc) gene is a target of the JUN N-terminal kinase signaling pathway during dorsal closure, we investigated puc gene expression during wound healing in Drosophila. We showed that puc gene expression is induced at the edge of the wound in epithelial cells and Jun kinase is phosphorylated in wounded epidermal tissues, suggesting that the JUN N-terminal kinase signaling pathway is activated by a signal produced by an epidermal wound. In the absence of the Drosophila c-Fos homologue, puc gene expression is no longer induced. Finally, impaired epithelial repair in JUN N-terminal kinase deficient flies demonstrates that the JUN N-terminal kinase signaling is required to initiate the cell shape change at the onset of the epithelial wound healing. We conclude that the embryonic JUN N-terminal kinase gene cassette is induced at the edge of the wound. In addition, Drosophila appears as a good in vivo model to study morphogenetic processes requiring epithelial regeneration such as wound healing in vertebrates. (C) 2001 Elsevier Science.