Brassica rapa orphan genes largely affect soluble sugar metabolism.
Brassica rapa orphan genes largely affect soluble sugar metabolism.
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油菜孤儿基因很大程度上影响可溶性糖代谢
DOI：
10.1038/s41438-020-00403-z
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发表时间：
2020-11-01
影响因子：
8.7
通讯作者：
Piao Z
中科院分区：
文献类型：
--
作者：
Jiang M;Zhan Z;Li H;Dong X;Cheng F;Piao Z
孤儿基因(OGs)是特定分类群特有的基因，在初级新陈代谢中起着至关重要的作用。然而，我们以前的研究中发现的Brassica Rapa OGs(BrOGs)的功能意义还知之甚少。为了研究它们的生物学功能，我们建立了一个包含43个基因的Brog过表达(BrOGOE)突变体文库，并对这些植物的表型变异进行了评估。我们发现43个BrOGOE突变体中有19个表现为突变表型，42个表现出可变的可溶性糖含量。选择了一个突变体BrOG1OE进行深入分析，该突变体的果糖、葡萄糖和总糖含量显著增加，但蔗糖含量降低。BrOG1OE降低了拟南芥蔗糖合成酶基因(AtSUS)的表达和活性，但转化酶活性没有变化。相反，BrOG1的两个拷贝BraA08002322(BrOG1A)和BraSca000221(BrOG1B)在大白菜(B.Rapa SSP.由于BrSUS1b、BrSUS3，特别是BrSUS5基因在编辑的BrOG1转基因株系中表达上调，导致果糖、葡萄糖和总可溶性糖含量降低。此外，我们还观察到BrOG1突变体的蔗糖含量和SUS活性增加，转化酶活性保持不变。因此，BrOG1可能以一种SUS依赖的方式影响可溶性糖代谢。这是第一个关于BrOGs在可溶性糖代谢方面的功能的研究报告，并强化了OGs是营养代谢的宝贵资源的观点。
Orphan genes (OGs), which are genes unique to a specific taxon, play a vital role in primary metabolism. However, little is known about the functional significance of Brassica rapa OGs (BrOGs) that were identified in our previous study. To study their biological functions, we developed a BrOG overexpression (BrOGOE) mutant library of 43 genes in Arabidopsis thaliana and assessed the phenotypic variation of the plants. We found that 19 of the 43 BrOGOE mutants displayed a mutant phenotype and 42 showed a variable soluble sugar content. One mutant, BrOG1OE, with significantly elevated fructose, glucose, and total sugar contents but a reduced sucrose content, was selected for in-depth analysis. BrOG1OE showed reduced expression and activity of the Arabidopsis sucrose synthase gene (AtSUS); however, the activity of invertase was unchanged. In contrast, silencing of two copies of BrOG1 in B. rapa, BraA08002322 (BrOG1A) and BraSca000221 (BrOG1B), by the use of an efficient CRISPR/Cas9 system of Chinese cabbage (B. rapa ssp. campestris) resulted in decreased fructose, glucose, and total soluble sugar contents because of the upregulation of BrSUS1b, BrSUS3, and, specifically, the BrSUS5 gene in the edited BrOG1 transgenic line. In addition, we observed increased sucrose content and SUS activity in the BrOG1 mutants, with the activity of invertase remaining unchanged. Thus, BrOG1 probably affected soluble sugar metabolism in a SUS-dependent manner. This is the first report investigating the function of BrOGs with respect to soluble sugar metabolism and reinforced the idea that OGs are a valuable resource for nutrient metabolism.
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影响因子：
11.6
作者：
Griffiths, Jayne;Murase, Kohji;Thomas, Stephen G.
通讯作者：
Thomas, Stephen G.
影响因子：
12.3
作者：
Lawrenson T;Shorinola O;Stacey N;Li C;Østergaard L;Patron N;Uauy C;Harwood W
通讯作者：
Harwood W
影响因子：
5.6
作者：
Li X;Huang L;Lu J;Cheng Y;You Q;Wang L;Song X;Zhou X;Jiao Y
通讯作者：
Jiao Y
DOI：
10.1073/pnas.0900689106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Barratt, D. H. Paul;Derbyshire, Paul;Smith, Alison M.
通讯作者：
Smith, Alison M.
影响因子：
7.4
作者：
Braatz, Janina;Harloff, Hans-Joachim;Jung, Christian
通讯作者：
Jung, Christian