Expression of Tandem P Domain K+ Channel, TREK-1, in the Rat Carotid Body
Expression of Tandem P Domain K+ Channel, TREK-1, in the Rat Carotid Body
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DOI：
10.1369/jhc.5a6755.2005
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发表时间：
2006-04
影响因子：
3.2
通讯作者：
Y. Yamamoto;K. Taniguchi
中科院分区：
文献类型：
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作者：
Y. Yamamoto;K. Taniguchi
TREK-1是调节细胞膜兴奋性的重要钾通道之一。为了检测TREK-1在大鼠颈动脉体中的分布，我们进行了TREK-1 mRNA表达的RT-PCR和原位杂交以及组织分布的免疫组织化学。RT-PCR检测颈动脉体中TREK-1 mRNA的表达。此外，原位杂交显示TREK-1 mRNA在血管球细胞中的定位。TREK-1主要分布于颈动脉体的球细胞和神经纤维。TREK-1可调节大鼠颈动脉体血管球细胞和/或传入神经末梢的钾电流。
TREK-1 is one of the important potassium channels for regulating membrane excitability. To examine the distribution of TREK-1 in the rat carotid body, we performed RT-PCR for mRNA expression and in situ hybridization and immunohistochemistry for tissue distribution of TREK-1. RT-PCR detected mRNA expression of TREK-1 in the carotid body. Furthermore, in situ hybridization revealed the localization of TREK-1 mRNA in the glomus cells. TREK-1 immunoreactivity was mainly distributed in the glomus cells and nerve fibers in the carotid body. TREK-1 may modulate potassium current of glomus cells and/or afferent nerve endings in the rat carotid body.