A simplified and efficient protocol for derivation and maintenance of high-quality mouse primed pluripotent stem cells using Wnt inhibition
A simplified and efficient protocol for derivation and maintenance of high-quality mouse primed pluripotent stem cells using Wnt inhibition
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使用 Wnt 抑制来衍生和维持高质量小鼠引发的多能干细胞的简化且有效的方案
DOI：
10.1002/cpsc.60
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Abe K.
中科院分区：
文献类型：
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作者：
Kondo M;Sugimoto M;Abe K.
外胚层干细胞（Epiblast stem cells, EpiSCs）是一种从小鼠移植后胚胎中提取的多能干细胞（PSCs）。有趣的是，EpiSCs与人类胚胎干细胞（hESCs）和人类诱导的psc （hiPSC）等人类psc具有许多共同的特征。因此，EpiSCs可以作为研究多能性启动状态的模型。本文描述了一种简单而高效的EpiSC衍生和使用WNT分泌抑制剂维持均质EpiSC的方案。使用该方法，EpiSCs可以从C57BL/6N等不同遗传背景的小鼠株中获得。通过该方案衍生的EpiSCs保持同质，未分化状态，但保留高分化潜力。与原始方案建立的EpiSC不同，新的EpiSC系需要持续存在WNT抑制剂，这表明与原始方法制备的EpiSC存在内在差异。这一新版本的EpiSCs将为理解哺乳动物多能细胞启动状态的本质提供线索，并可能有助于建立更好的从启动状态定向分化的方案。©2018 by John Wiley & Sons, Inc。
Epiblast stem cells (EpiSCs) are primed pluripotent stem cells (PSCs) derived from mouse postimplantation embryos. Interestingly, EpiSCs share many characteristics with human PSCs such as human embryonic stem cells (hESCs) and human induced PSCs (hiPSC). Thus, EpiSCs can serve as a model for studying primed states of pluripotency. This article describes a simple yet highly efficient protocol for EpiSC derivation and maintenance of homogenous EpiSCs using an inhibitor of WNT secretion. Using this method, EpiSCs can be readily derived from mouse strains with different genetic background including C57BL/6N. The EpiSCs derived by this protocol maintain a homogenous, undifferentiated status, yet retain high differentiation potential. Unlike EpiSCs established by the original protocol, the new EpiSC lines require the continued presence of WNT inhibitor, suggesting intrinsic differences from EpiSCs made by the original method. This new version of EpiSCs will provide clues to understand the nature of primed states of mammalian pluripotent cells and may facilitate establishment of a better protocol for directed differentiation from the primed state. © 2018 by John Wiley & Sons, Inc.