The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled
The Glide/Gcm fate determinant controls initiation of collective cell migration by regulating Frazzled
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DOI：
10.7554/elife.15983
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发表时间：
2016-10-14
期刊：
影响因子：
7.7
通讯作者：
Giangrande, Angela
中科院分区：
文献类型：
--
作者：
Gupta, Tripti;Kumar, Arun;Giangrande, Angela
集体迁移是一个复杂的过程，有助于建立精确的组织和器官结构。与细胞相互作用有关的几个分子也控制着集体迁移，但它们的确切作用和协调这一复杂发育过程的精细表达尚不清楚。在这里，我们证明了Netrin受体Frazzed的及时和阈值表达触发了发育中的果蝇翅膀中胶质细胞迁移的启动。转录因子Glide/GCM以剂量依赖的方式诱导疲劳表达。因此，神经胶质决定因素也调节集体迁徙的效率。NETRINB而不是NETRINA是一种趋化物质，而UNC5则是一种排斥神经胶质细胞迁移的NETRIN受体。我们的模型包括对配体、细胞自主作用的受体和命运决定因素的严格空间定位，这些决定因素协同作用，将神经胶质细胞引向其最终目的地。
Collective migration is a complex process that contributes to build precise tissue and organ architecture. Several molecules implicated in cell interactions also control collective migration, but their precise role and the finely tuned expression that orchestrates this complex developmental process are poorly understood. Here, we show that the timely and threshold expression of the Netrin receptor Frazzled triggers the initiation of glia migration in the developing Drosophila wing. Frazzled expression is induced by the transcription factor Glide/Gcm in a dose-dependent manner. Thus, the glial determinant also regulates the efficiency of collective migration. NetrinB but not NetrinA serves as a chemoattractant and Unc5 contributes as a repellant Netrin receptor for glia migration. Our model includes strict spatial localization of a ligand, a cell autonomously acting receptor and a fate determinant that act coordinately to direct glia toward their final destination.