ANTIBODY-CATALYZED DEGRADATION OF COCAINE
ANTIBODY-CATALYZED DEGRADATION OF COCAINE
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DOI：
10.1126/science.8456315
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发表时间：
1993-03-26
期刊：
影响因子：
56.9
通讯作者：
GEORGIADIS, TM
中科院分区：
文献类型：
--
作者：
LANDRY, DW;ZHAO, K;GEORGIADIS, TM
用一种类似于可卡因的过渡态膦酸单酯免疫，可以获得能够催化可卡因苯甲酰酯基水解的单克隆抗体。放射性标记可卡因的降解试验鉴定出了活性酶。苯甲酰酯水解产生卵黄碱甲酯和苯甲酸，这些碎片缺乏可卡因的刺激活性。这种人工酶的被动免疫可以通过钝化强化来治疗依赖性。
Immunization with a phosphonate monoester transition-state analog of cocaine provided monoclonal antibodies capable of catalyzing the hydrolysis of the cocaine benzoyl ester group. An assay for the degradation of radiolabeled cocaine identified active enzymes. Benzoyl esterolysis yields ecgonine methyl ester and benzoic acid, fragments devoid of cocaine's stimulant activity. Passive immunization with such an artificial enzyme could provide a treatment for dependence by blunting reinforcement.