Identification of amino-acid residues in the V protein of peste des petits ruminants essential for interference and suppression of STAT-mediated interferon signaling
Identification of amino-acid residues in the V protein of peste des petits ruminants essential for interference and suppression of STAT-mediated interferon signaling
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DOI：
10.1016/j.virol.2015.03.039
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发表时间：
2015-09-01
期刊：
影响因子：
3.7
通讯作者：
Cai, Xuepeng
中科院分区：
文献类型：
--
作者：
Ma, Xusheng;Yang, Xing;Cai, Xuepeng
小反刍兽疫病毒（PPRV）是一种可引起小型反刍动物死亡的病毒。PPRV的V蛋白通过与STAT 1和STAT 2相互作用阻断IFN信号通路，在干扰宿主天然免疫中发挥重要作用。本研究结果表明，PPRV V蛋白以剂量依赖的方式阻断IFN的作用，并抑制STAT 1/2蛋白的转位。我们推测这种易位抑制可能是通过干扰STAT蛋白的下游而引起的。突变确定PPRV V蛋白的Cys簇和Trp基序是STAT介导的IFN信号转导所必需的。这些发现为进一步研究PPRV逃避IFN信号转导的机制提供了新的视角。(C)2015 Elsevier Inc. All rights reserved.
Peste des petits ruminants virus (PPRV) causes a fatal disease in small ruminants. V protein of PPRV plays a pivotal role in interfering with host innate immunity by blocking IFNs signaling through interacting with STAT1 and STAT2. In the present study, the results demonstrated that PPRV V protein blocks IFN actions in a dose dependent manner and restrains the translocation of STAT1/2 proteins. We speculate that the translocation inhibition might be caused by the interfering of the downstream of STAT protein. Mutagenesis defines that Cys cluster and Trp motif of PPRV V protein are essential for STAT-mediated IFN signaling. These findings give a new sight for the further studies to understand the delicate mechanism of PPRV to escape the IFN signaling. (C) 2015 Elsevier Inc. All rights reserved.