Glut1 and Glut3 expression, but not capillary density, is increased by cobalt chloride in rat cerebrum and retina
Glut1 and Glut3 expression, but not capillary density, is increased by cobalt chloride in rat cerebrum and retina
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DOI：
10.1016/s0169-328x(98)00301-5
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发表时间：
1999-01-22
期刊：
影响因子：
--
通讯作者：
Ismail-Beigi, F
中科院分区：
文献类型：
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作者：
Badr, GA;Zhang, JZ;Ismail-Beigi, F
氯化钴[Co(Li)]是一种刺激一组低氧反应基因表达的试剂，经10-12天处理后，大鼠大脑灰质中Glut1mRNA和Glut1的含量分别增加了1.45倍和1.40倍(P<；0.05)。Glut1含量的增加与大脑灰质分离的微血管中Glut1染色的含量显著增加以及额叶和海马区微血管中Glut1免疫组织化学染色强度的增加有关。Co(II)组大鼠大脑中GLUT3的丰度也增加了1.3倍(P<；0.05)，但这种增加与GLUT3mRNA含量的变化无关。在视网膜，经Co(II)处理后，Glut1mRNA和Glut1蛋白的含量分别增加了2.48倍和1.23倍(P<；0.05)；在分离的视网膜微血管中，Glut1蛋白的表达也有类似的增加。钴(II)处理组大鼠视网膜GLUT3含量增加1.5倍(P<；0.05)。此外，经Co(II)处理后，大鼠大脑中血管内皮生长因子的表达显著增加2.2倍。然而，尽管Co(II)诱导Glut1在脑微血管和视网膜微血管中的表达增加，以及在大脑中的VEGF表达增加，但这两种组织的毛细血管密度都没有增加。结果表明，暴露于Co(II)10-12天后，大脑皮层和视网膜中两种主要葡萄糖转运蛋白的表达增强，但不增加两者的毛细血管密度，这可能是通过缺氧信号途径起作用的。(C)1999 Elsevier Science B.V.保留所有权利。
Treatment of rats with cobalt chloride [Co(LI)], an agent that stimulates the expression of a set of hypoxia-responsive genes, for 10-12 days resulted in 1.45- and 1.40-fold increases in the content of Glut1 mRNA and Glut1 in cerebral gray matter, respectively (P < 0.05 for both changes). The increase in Glut1 content was associated with a significant increase in the content of Glut1 staining in microvessels isolated from cerebral gray matter, and in the intensity of Glut1 in microvessels of the frontal lobe and hippocampus assessed by immunohistochemistry. The abundance of Glut3 in cerebrum of Co(II)-treated rats also increased by 1.3-fold(P < 0.05), but the increase was not associated with a change in the content of Glut3 mRNA. In retina, treatment with Co(II) resulted in 2.48- and 1.23-fold increases in the content of Glut1 mRNA and Glut1 protein, respectively (P < 0.05 for both changes); similar increases in Glut1 protein expression were observed in isolated retinal microvasculature. The content of Glut3 in retina also increased 1.5-fold in Co(II)-treated rats (P < 0.05). In addition, treatment with Co(II) resulted in a significant 2.2-fold increase in the expression of VEGF in the cerebrum. However, despite the Co(II)-induced increase in Glut1 expression in cerebral and retinal microvasculature and VEGF in cerebrum, there was no increase in the capillary density in either tissue. It is concluded that a 10-12 day exposure to Co(II), presumably acting through the hypoxia-signaling pathway, results in enhanced expression of both major glucose transporters in cerebral cortex and retina, without increasing the capillary density of either tissue. (C) 1999 Elsevier Science B.V. All rights reserved.