Mammalian spermatogenesis in vivo and in vitro: a partnership of spermatogenic and somatic cell lineages.
Mammalian spermatogenesis in vivo and in vitro: a partnership of spermatogenic and somatic cell lineages.
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DOI：
10.1210/edrv-15-1-116
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发表时间：
1994-02
影响因子：
20.3
通讯作者：
A. Kierszenbaum
中科院分区：
文献类型：
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作者：
A. Kierszenbaum
体外配子发生可能吗？这个问题是1965年Etienne Wolff和凯蒂·哈芬（1）发表的一篇综合性综述中的相关主题之一，该综述描述了1913年至1965年期间在脊椎动物和无脊椎动物性腺上进行的开创性器官培养工作的结果。Wolff和Haffen对上述问题提供了部分答案，指出器官培养方法允许“生殖细胞发生更大的分化，因为它们被其自然环境，即性腺的体细胞所包围”。早期认识到生精细胞可以在体外与体细胞密切相关地分化，可以被视为本声明最具前瞻性的方面。Wolff和Haffen的综述中的另一个重要评论是体外器官和细胞培养系统的预期潜在用途，用于确定包括激素在内的各种因素在精子发生过程调节中的作用。
Is gametogenesis possible in vitro? This question was one of the relevant topics in a comprehensive review published in 1965 by Etienne Wolff and Katy Haffen (1), describing results of pioneering organ culture work on vertebrate and invertebrate gonads carried out between 1913 and 1965. Wolff and Haffen provided a partial answer to the above question by indicating that organ culture methods allow “far greater differentiation of the germ cells to occur since they are surrounded by their natural environment, namely the somatic cells of the gonads.” The early recognition that spermatogenic cells can differentiate in vitro in close association with somatic cells can be regarded as the most forwardlooking aspect of this statement. Another important remark in Wolff and Haffen's review was the anticipated potential use of in vitro organ and cell culture systems for determining the role of various factors, including hormones, in the regulation of the spermatogenic process.