The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.
The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.
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DOI：
10.1101/gad.6.9.1716
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发表时间：
1992-09
影响因子：
10.5
通讯作者：
Weidong Wang;J. Gralla;Michael F. Carey
中科院分区：
文献类型：
--
作者：
Weidong Wang;J. Gralla;Michael F. Carey
激活的RNA聚合酶II（pol II）转录复合物的组装已被研究，通过分析是否预组装的中间复合物减少了延长的起始位点熔化所需的时间。结果表明，一个封闭的复合物需要因子IIA，IID，和酸性激活剂GAL 4-AH的形式在限速步骤。这引导模板进入生产性组装途径。然后迅速加入因子TFIIB，提供进一步的保护，防止转移到非生产性途径。这些事件之后是一系列快速步骤，其中剩余的一般因子组装到模板上，然后使用ATP水解的能量将其熔化。
The assembly of activated RNA polymerase II (pol II) transcription complexes has been investigated by assaying whether pre-assembly of intermediate complexes reduces the extended time required for start-site melting. The results show that a closed complex requiring factors IIA, IID, and the acidic activator GAL4-AH forms in a rate-limiting step. This directs the templates into a productive assembly pathway. Factor TFIIB is then added rapidly, affording further protection against diversion into nonproductive pathways. These events are followed by a series of rapid steps in which the remaining general factors are assembled onto the template, which is then melted using the energy of ATP hydrolysis.