MALDI Mass Spectrometry Imaging of Biological Structures
MALDI Mass Spectrometry Imaging of Biological Structures
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生物结构的 MALDI 质谱成像
DOI：
10.1002/9780470027318.a9417
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Takahiro Hayasaka
中科院分区：
文献类型：
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作者：
Nobuhiro Zaima;Naoko Goto-Inoue;Takahiro Hayasaka
基质辅助激光解吸/电离（MALDI）是一种“软”电离方法，可检测生物样品中的多种分子，包括蛋白质、肽、氨基酸、糖、脂质和代谢物。MALDI的多功能性使其成为检测复杂生物结构中感兴趣分子的必要分析方法。为了利用MALDI的这一优势，基质辅助激光解吸/电离质谱成像（MALDI-MSI）已被开发为一种分子成像方法，用于可视化生物样品切片中分子的空间分布。据报道，几乎所有类型的分子，如生物分子和施用的药物或食物因子，在生物样品中可以通过MALDI-MSI可视化。由于这些分子中的许多分子除了MALDI-MSI之外无法可视化，因此MALDI-MSI在医学科学，制药科学，植物科学和食品科学等多个领域开辟了新的前沿。为了获得感兴趣的分子分布的生物学意义的信息，研究人员应该了解MALDI-MSI的基本实验步骤和潜在应用。本文介绍了MALDI-MSI的原理、基本实验步骤和应用。
Matrix‐assisted laser desorption/ionization (MALDI) is a ‘soft’ ionization method which enables the detection of several molecules including proteins, peptides, amino acids, sugars, lipids, and metabolites, in biological samples. The versatility of MALDI makes it essential analytical method to detect the molecules of interest in the complex biological structures. To take this advantage of MALDI, matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI) has been developed as a molecular imaging method to visualize the spatial distribution of molecules in biological sample section. It has been reported that almost all kinds of molecules, such as biomolecules and administrated drugs or food factors, in biological samples can be visualized by MALDI‐MSI. Because many of these molecules cannot be visualized with the exception of MALDI‐MSI, MALDI‐MSI opens a new frontier in several fields such as medical science, pharmaceutical science, plant science, and food science. To obtain biologically meaningful information of the distribution of molecules of interest, researcher should understand the basic experimental steps and potential applications of MALDI‐MSI. In this article, principle, basic experimental procedure, and applications of MALDI‐MSI are described.