Covalent Immobilization of Collagen on Titanium through Polydopamine Coating to Improve Cellular Performances of MC3T3-E1 Cells.
Covalent Immobilization of Collagen on Titanium through Polydopamine Coating to Improve Cellular Performances of MC3T3-E1 Cells.
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DOI：
10.1039/c3ra44137g
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发表时间：
2013-11-21
期刊：
影响因子：
3.9
通讯作者：
Wei M
中科院分区：
文献类型：
--
作者：
Yu X;Walsh J;Wei M
骨科植入物的表面改性对于提高这些医疗器械的临床性能至关重要。在此，胶原蛋白共价固定到钛种植体表面上通过一种新的粘附聚多巴胺涂层的灵感来自贻贝粘附蛋白。采用X射线光电子能谱（XPS）和扫描电子显微镜（SEM）对胶原涂层的形成和组成进行表征。荧光标记的胶原蛋白也用于检查胶原蛋白涂层的形成和均匀性。与胶原蛋白的物理吸附相比，具有聚多巴胺支撑基底的所得胶原蛋白涂层在钛表面上表现出更好的均匀性和分布。共价固定的胶原蛋白涂层具有生物活性，如其增强MC 3 T3-E1细胞粘附、支持细胞增殖和促进前成骨细胞的早期成骨分化的能力所证明的。我们的研究表明，通过聚多巴胺涂层共价固定胶原蛋白可能是一种有效的方法，以提高种植体表面的细胞性能。
Surface modification of orthopedic implants is critical for improving the clinical performance of these medical devices. Herein, collagen was covalently immobilized onto a titanium implant surface via a novel adherent polydopamine coating inspired by mussel adhesive proteins. The formation and composition of the collagen coating was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Fluorescent labeled collagen was also used to examine the formation and uniformity of the collagen coating. The resultant collagen coating with a polydopamine supporting substrate demonstrated better uniformity and distribution on the titanium surface compared to a physical adsorption of collagen. The covalent immobilized collagen coating is biologically active, as evidenced by its ability to enhance MC3T3-E1 cell adhesion, support cell proliferation and promote early stage osteogenic differentiation of pre-osteoblasts. Our study suggests covalent immobilization of collagen through the polydopamine coating might be an efficient way to improve the cellular performance of implant surfaces.