The role of human papillomavirus in the increased incidence of base of tongue cancer
The role of human papillomavirus in the increased incidence of base of tongue cancer
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DOI：
10.1002/ijc.24994
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发表时间：
2010-06-15
影响因子：
6.4
通讯作者：
Munck-Wikland, Eva
中科院分区：
文献类型：
--
作者：
Attner, Per;Du, Juan;Munck-Wikland, Eva
许多报告表明，口咽癌的发病率正在增加，而人类乳头瘤病毒(HPV)是一个危险因素。然而，很少有研究对口咽部的特定亚群进行研究。根据我们之前对扁桃体癌的研究，我们评估了舌根癌发病率的增加和这种疾病中HPV的流行。在1998至2007年间，斯德哥尔摩县有109名患者被诊断为舌根癌。从患者的95例石蜡包埋的肿瘤活检组织中获得HPV，并用普通HPV聚合酶链式反应和HPV-16/HPV-33型特异性聚合酶链式反应对其进行检测。检测HPV-16RNA表达以确认E6和/或E7的表达。发病率数据从瑞典癌症登记处获得。瑞典的舌癌发病率总体上从1970-1974年的0.15/100,000人年增加到2005-2007年的0.47/100,000人年。斯德哥尔摩县舌癌根部的HPV感染率从1998-2001年的58%上升到2004-2007年的84%(p<；0.05)。在HPV阳性的肿瘤中，HPV-16占优势(86%)，但有趣的是，HPV33的检出率高达10%。在检测的样本中有85%检测到E6和/或E7RNA。在研究期间，瑞典舌癌根部的发病率以及HPV阳性肿瘤的比例都有所增加，这表明HPV可能是导致这一增长的原因。
Numerous reports have shown that the incidence for oropharyngeal cancer is increasing and that human papillomavirus (HPV) is a risk factor. However, few studies have investigated the specific subsites of the oropharynx. Following our previous research on tonsillar cancer, we assessed the increase in the incidence of base of tongue cancer and the prevalence of HPV in this disease. Between 1998 and 2007, 109 patients were diagnosed for base of tongue cancer in Stockholm county. Ninety-five paraffin-embedded diagnostic tumor biopsies from patients were obtained and tested for HPV, both by general HPV PCR and HPV-16/HPV-33 type-specific PCR. Expression of HPV-16 RNA was analyzed to confirm E6 and/or E7 expression. Incidence data were obtained from the Swedish Cancer Registry. An overall increase in the incidence of base of tongue cancer from 0.15/100,000 person-years during 1970-1974 to 0.47/100,000 person-years during 2005-2007 was found in Sweden. The prevalence of HPV in base of tongue cancer in Stockholm county increased from 58% during 1998-2001 to 84% during 2004-2007 (p < 0.05). In the HPV-positive tumors, HPV-16 dominated (86%) but interestingly, HPV33 was detected in as many as 10%. E6 and/or E7 RNA were found in 85% of the samples tested. The incidence of base of tongue cancer, as well as the proportion of HPV-positive tumors, has increased in Sweden during the study period, suggesting that HPV may contribute to this increase.