Manganese-enhanced MRI reflects seizure outcome in a model for mesial temporal lobe epilepsy
Manganese-enhanced MRI reflects seizure outcome in a model for mesial temporal lobe epilepsy
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DOI：
10.1016/j.neuroimage.2012.11.054
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发表时间：
2013-03-01
期刊：
影响因子：
5.7
通讯作者：
O'Brien, T. J.
中科院分区：
文献类型：
--
作者：
Dedeurwaerdere, S.;Fang, K.;O'Brien, T. J.
导致癫痫的神经生物学过程，称为癫痫发生，目前还不完全清楚。锰增强磁共振成像(MEMRI)可能有助于这一探索，因为它提供了优越的组织对比度，特别是海马区的对比度。为探讨海人酸致癫痫持续状态(KASE)后大鼠内侧颞叶癫痫模型的MEMRI动态变化，分别于KASE(10~30 mg/kg，ip)前、后2天和6周采集MEMRI图像。14只大鼠和11只对照组大鼠，而第二组对照组(N=6)和癫痫动物(N=10)仅在KASE后2个月进行成像。扫描前1d右侧脑室注射氯化锰(50 mm，10亩L)。在海马区和海马区的几个亚区(CA1、CA3和齿状回)周围画出了感兴趣的区域。对自发性反复发作、海马神经元丢失、苔藓纤维发芽等癫痫标志物进行量化。与对照组相比，KASE组大鼠海马区、CA1区和齿状回的MEMRI信号强度持续增强(ANOVA P
The neurobiological processes resulting in epilepsy, known as epileptogenesis, are incompletely understood. Manganese-enhanced MRI (MEMRI) can potentially aide in this quest as it provides superior tissue contrast, particularly of the hippocampal subregions. This longitudinal study aims to characterise the changes in the hippocampus of the post kainic acid-induced status epilepticus (KASE) rat model of mesial temporal lobe epilepsy using MEMRI in vivo.Serial acquisition of T-1-weighted MEMRI images were taken before, 2 days and 6 weeks after KASE (10-30 mg/kg, i.p.) in 14 rats and in 11 control rats, while a second cohort of control (N=6) and epileptic animals (N=10) was imaged at 2 months post KASE only. MnCl2 (50 mM, 10 mu l) was administered in the right lateral ventricle 1 day before scanning. Regions of interest were drawn around the hippocampus and several subregions of the hippocampus (CA1, CA3 and dentate gyrus). Markers of epilepsy such as spontaneous recurrent seizures, hippocampal neuronal loss and mossy fiber sprouting were quantified. A persistent increase in MEMRI signal intensity was found in the hippocampus, CA1 and dentate gyrus in the KASE group compared to the control group (ANOVA P