The relationship between cyclooxygenase-2 expression and colorectal cancer
The relationship between cyclooxygenase-2 expression and colorectal cancer
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DOI：
10.1001/jama.282.13.1254
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发表时间：
1999-10-06
影响因子：
120.7
通讯作者：
Murray, FE
中科院分区：
文献类型：
--
作者：
Sheehan, KM;Sheahan, K;Murray, FE
背景流行病学研究表明环氧合酶-2(COX-2)在结直肠癌的发病机制中起重要作用，但其作用尚不完全清楚。目的探讨COX-2在结直肠癌组织中的表达与患者生存的关系。用兔抗人COX-2多克隆抗体对肿瘤切片进行COX-2染色，由2名观察者盲法对COX-2染色的程度进行分级，用COX-2多肽预先吸收抗COX-2抗体取消了染色，显示了该分析的特异性。Dukes D，n=12)，以14例正常结肠活检组织COX-2染色作为对照。主要观察指标COX-2上皮细胞染色程度(1级，
Context Epidemiological studies have implicated the inducible form of cyclooxygenase (COX-2) in the pathogenesis of colorectal cancer; however, its role is not fully understood.Objective To examine the relationship between the expression of COX-2 in human colorectal cancer and patient survival.Design Patients diagnosed as having colorectal cancer were evaluated and followed up for up to 9.4 years (median follow-up, 2.7 years). Tumor sections were stained for COX-2 using a rabbit polyclonal antibody raised against human COX-2, The extent of COX-2 staining was graded by 2 observers blinded to outcome, Preabsorption of the anti-COX-2 antibody with a COX-2 peptide abolished the staining, demonstrating the specificity of the assay.Setting Gastrointestinal unit of a large general teaching hospital in Dublin, Ireland.Participants Seventy-six patients (median age, 66.5 years) with colorectal cancer (Dukes tumor stage A, n = 9; Dukes B, n = 30; Dukes C, n = 25; Dukes D, n = 12) whose diagnosis was made between 1988 and 1991, Fourteen normal colon biopsies were stained for COX-2 as controls.Main Outcome Measures Survival in years following diagnosis compared by extent of COX-2 epithelial staining (grade 1,