Molecular cloning, chromosome mapping and characterization of the mouse CRTH2 gene, a putative member of the leukocyte chemoattractant receptor family
Molecular cloning, chromosome mapping and characterization of the mouse CRTH2 gene, a putative member of the leukocyte chemoattractant receptor family
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DOI：
10.1016/s0378-1119(98)00599-x
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发表时间：
1999-02-04
期刊：
影响因子：
3.5
通讯作者：
Sugamura, K
中科院分区：
文献类型：
--
作者：
Abe, H;Takeshita, T;Sugamura, K
我们克隆了小鼠 CRTH2（在 TH2 细胞上表达的趋化受体同源分子）基因，该基因编码假定的白细胞趋化受体，其人类同源物在 T 细胞克隆中的 Th2 克隆中选择性表达，但在 Th1 克隆中不表达。推导的小鼠CRTH2氨基酸序列与其人类同源物具有77%的同一性。系统发育分析表明，小鼠和人CRTH2均与白细胞趋化受体中的N-甲酰肽受体和C5a受体密切相关。通过 FISH 将小鼠 CRTH2 基因定位在 19c 号染色体上，其中没有定位白细胞趋化受体的其他基因。 RT-PCR 分析显示小鼠 CRTH2 mRNA 在多种细胞系中表达，包括造血细胞系和非造血细胞系。在肝、肺、肾、脑、心脏、胸腺和脾中也观察到表达。这些结果表明小鼠 CRTH2 在多种细胞中发挥作用，使得 CRTH2 的作用具有多效性。 (C) 1999 Elsevier Science B.V. 保留所有权利。
We have cloned the mouse CRTH2 (chemoattractant receptor-homologous molecule expressed on TH2 cells) gene encoding a putative leukocyte chemoattractant receptor, of which human homologue is expressed selectively in Th2 but not in Th1 clones among T cell clones. The deduced amino-acid sequence of mouse CRTH2 bears 77% identity with its human homologue. Phylogenetic analysis suggested that both mouse and human CRTH2 are closely related to the N-formyl peptide receptor and the C5a receptor among leukocyte chemoattractant receptors. The mouse CRTH2 gene was mapped on chromosome 19c with FISH, where no other genes for leukocyte chemoattractant receptors are mapped. RT-PCR analysis revealed that mouse CRTH2 mRNA is expressed in various cell lineages, including both hematopoietic and non-hematopoietic cell lines. Expression was also observed in liver, lung, kidney, brain, heart, thymus, and spleen. These results suggest that mouse CRTH2 functions in a variety of cells, making the effects of CRTH2 pleiotropic. (C) 1999 Elsevier Science B.V. All rights reserved.