Differential localization of the LIM domain protein PLIM-1 in microspores and mature pollen drains from sunflower
Differential localization of the LIM domain protein PLIM-1 in microspores and mature pollen drains from sunflower
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DOI：
10.1007/s004970050172
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发表时间：
1999-03-01
影响因子：
--
通讯作者：
Steinmetz, A
中科院分区：
文献类型：
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作者：
Baltz, R;Schmit, AC;Steinmetz, A
PLIM-1是在花粉粒中特异表达的LIM结构域蛋白。利用两个PLIM-1特异性单克隆抗体，我们研究了其在向日葵（Helianthus annuus L.）花粉我们的研究表明，蛋白质出现在小孢子阶段的细胞质体的数量有限。在双细胞花粉中检测不到，并在皮层斑块中的三细胞花粉粒中重新出现，特别是集中在营养细胞的富含F-肌动蛋白的萌发锥中。发育阶段依赖性，不同的位置的蛋白质表明在花粉发育过程中的双重功能。虽然这种功能在小孢子发育仍然模糊，高浓度的PLIM-1在成熟花粉的萌发锥表明，它参与了萌发过程，以及在花粉管生长。
PLIM-1 is a LIM domain protein specifically expressed in pollen grains. Using two PLIM-1-specific monoclonal antibodies we studied its expression and intracellular location at various developmental stages of sunflower (Helianthus annuus L.) pollen. Our studies show that the protein appears at the microspore stage in a limited number of cytoplasmic bodies. becomes undetectable in bicellular pollen, and reappears in tricellular pollen grains in cortical patches particularly concentrated in the F-actin-enriched germination cones of the vegetative cell. The developmental stage-dependent, different location of the protein suggests a dual function during pollen development. While this function in microspore development remains obscure, the high concentration of PLIM-1 in the germination cones of mature pollen suggests that it participates in the germination process as well as in pollen tube growth.