The glypican Dally-like is required for Hedgehog signalling in the embryonic epidermis of Drosophila
The glypican Dally-like is required for Hedgehog signalling in the embryonic epidermis of Drosophila
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DOI：
10.1242/dev.00874
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Sanson, B
中科院分区：
文献类型：
--
作者：
Desbordes, SC;Sanson, B
果蝇基因dally和dally-like编码磷脂酰肌醇聚糖，其是通过糖基磷脂酰肌醇连接锚定到细胞膜的硫酸乙酰肝素蛋白聚糖。遗传学研究表明，Dally和Dally like在胚胎和成虫盘中的无翅信号中起作用。在这里，我们测试这些分子在胚胎表皮中的信号特性。我们证明，RNA干扰沉默的dally样，但不是dally，给出了一个片段极性表型相同的无效突变无翅或刺猬。利用胚胎中的异源表达，我们解偶联刺猬和无翅信号通路，并发现Dally样和Dally，单独或一起，是没有必要的无翅信号。然而，Dally样对于Hedgehog信号转导是严格必需的。上位性实验表明，Dally样是必需的刺猬信号的接收，上游或在补丁受体的水平。
The Drosophila genes dally and dally-like encode glypicans, which are heparan sulphate proteoglycans anchored to the cell membrane by a glycosylphosphatidylinositol link. Genetic studies have implicated Dally and Dally-like in Wingless signalling in embryos and imaginal discs. Here, we test the signalling properties of these molecules in the embryonic epidermis. We demonstrate that RNA interference silencing of dally-like, but not dally, gives a segment polarity phenotype identical to that of null mutations in wingless or hedgehog. Using heterologous expression in embryos, we uncoupled the Hedgehog and Wingless signalling pathways and found that Dally-like and Dally, separately or together, are not necessary for Wingless signalling. Dally-like, however, is strictly necessary for Hedgehog signal transduction. Epistatic experiments show that Dally-like is required for the reception of the Hedgehog signal, upstream or at the level of the Patched receptor.