Regulation of the dynamic RNA Pol II elongation rate in Drosophila embryos.
Regulation of the dynamic RNA Pol II elongation rate in Drosophila embryos.
复制标题
果蝇胚胎中动态 RNA Pol II 伸长率的调节。
DOI：
10.1016/j.celrep.2023.113225
复制
发表时间：
2023
期刊：
影响因子：
8.8
通讯作者：
Lim,Bomyi
中科院分区：
文献类型：
--
作者：
Keller,SamuelH;Deng,Hao;Lim,Bomyi
越来越多的研究表明，RNA聚合酶II（RNA Pol II）延伸在基因调控中起着关键作用。我们系统地研究了各种增强子，启动子和基因体组成如何影响RNA Pol II的延伸率，通过单细胞分辨率活成像分析。通过使用含有5′ MS 2和3′ PP 7重复茎环的报告基因构建体，我们定量了活果蝇胚胎中RNA Pol II延伸的速率。我们发现启动子和外显子基因长度对延伸率没有影响，而增强子和长内含子的存在可能会显着改变RNA Pol II在基因中移动的速度。此外，我们在多个构建体中观察到，RNA Pol II延伸速率在果蝇胚胎核周期14转录起始后加速。我们的研究提供了影响动态RNA Pol II延伸速率的各种机制的单细胞视图，最终影响mRNA产生速率。
An increasing number of studies have shown the key role that RNA polymerase II (RNA Pol II) elongation plays in gene regulation. We systematically examine how various enhancers, promoters, and gene body composition influence the RNA Pol II elongation rate through a single-cell-resolution live imaging assay. By using reporter constructs containing 5′ MS2 and 3′ PP7 repeating stem loops, we quantify the rate of RNA Pol II elongation in liveDrosophilaembryos. We find that promoters and exonic gene lengths have no effect on elongation rate, while enhancers and the presence of long introns may significantly change how quickly RNA Pol II moves across a gene. Furthermore, we observe in multiple constructs that the RNA Pol II elongation rate accelerates after the transcriptional onset of nuclear cycle 14 inDrosophilaembryos. Our study provides a single-cell view of various mechanisms that affect the dynamic RNA Pol II elongation rate, ultimately affecting the rate of mRNA production.