Preformed angiotensin II is present in human mast cells
Preformed angiotensin II is present in human mast cells
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DOI：
10.1007/s10557-004-6218-y
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发表时间：
2004-11-01
影响因子：
3.4
通讯作者：
Matsumori, A
中科院分区：
文献类型：
--
作者：
Hara, M;Ono, K;Matsumori, A
目的：心力衰竭患者心肌肥大细胞密度增加。然而，其功能仍不清楚。在这项研究中，预先形成的血管紧张素II（ANG II），一种有效的生长因子，被发现包含在人肥大细胞中，并由肥大细胞释放。方法：将人肥大细胞系（HMC-1）与0 ~ 10（-6）M降钙素基因相关肽（CGRP）或培养液共同孵育。RT-PCR检测血管紧张素系统mRNA的表达。用ELISA和抗人ANG Ⅱ单克隆抗体免疫组化法检测HMC-1中ANG Ⅱ的存在。结果：ELISA和免疫组化法检测到人肥大细胞系（HMC-1）中存在血管紧张素Ⅱ（ANG Ⅱ）的表达。聚合酶链反应证实HMC-1中存在血管紧张素原和肾素的mRNA，而血管紧张素转换酶（ACE）的mRNA检测不到。由于心肌肥大细胞与神经纤维连接并在功能上与CGRP相关，因此检查了CGRP对HMC-1释放ANG II的影响。CGRP可诱导HMC-1细胞释放ANG Ⅱ，并增加血管紧张素原mRNA的表达。结论：人肥大细胞中存在预先形成的ANG Ⅱ，并有肾素-血管紧张素系统基因的表达。肥大细胞中ANG Ⅱ的释放和合成受CGRP的调节。
Purpose: The density of mast cells increases in the myocardium of patients suffering from heart failure. However, their function remains unclear. In this study, preformed angiotensin II (ANG II), a potent growth factor, was found to be contained in, and released by, human mast cells. \ Methods: The human mast cell line (HMC-1) was incubated with 0 to 10(-6) M calcitonin gene- related peptide ( CGRP) or culture medium. The expression of reninangiotensin system mRNA was examined using RT-PCR analysis. ELISA and immunohistochemistry with monoclonal antibody against human ANG II were performed to detect the presence of ANG II in HMC-1. The effect of CGRP on the expression of angiotensinogen mRNA was examined by quantitative RT-PCR analysis.Results: Preformed ANG II was detected in a human mast cell line ( HMC-1) which is a neoplastic cell line of mast cells by ELISA and immunohistochemistry. Presence of mRNA of angiotensinogen and renin was confirmed by polymerase chain reaction in HMC-1, while mRNA of angiotensin converting enzyme ( ACE) was undetectable. Since myocardial mast cells are interfaced with nerve fibers and functionally associated with CGRP, the effect of CGRP on ANG II release from HMC-1 was examined. CGRP induced the release of ANG II and increased angiotensinogen mRNA in HMC-1.Conclusions: The presence of preformed ANG II and gene expression of the renin-angiotensin system were detected in human mast cells. The release and synthesis of ANG II in mast cells was regulated by CGRP.