Plants expressing a miR164-resistant CUC2 gene reveal the importance of post-meristematic maintenance of phyllotaxy in Arabidopsis
Plants expressing a miR164-resistant CUC2 gene reveal the importance of post-meristematic maintenance of phyllotaxy in Arabidopsis
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DOI：
10.1242/dev.02774
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发表时间：
2007-03-15
期刊：
影响因子：
4.6
通讯作者：
Laufs, Patrick
中科院分区：
文献类型：
--
作者：
Peaucelle, Alexis;Morin, Halima;Laufs, Patrick
在植物中，器官沿茎的排列（叶序）遵循可预测的模式。最近的研究表明，分生组织的原基位置受局部生长素梯度控制，但对导致沿成熟茎叶序的后续事件知之甚少。我们在此表明，尽管分生组织的器官起始模式正常，但表达 miR164 抗性 CUP-SHAPED COTYLEDON2 (CUC2) 基因的植物在完全生长的茎中具有异常的叶序模式。这意味着茎生长过程中产生了异常的叶序。这些植物在茎中异位表达 CUC2，表明需要适当的 CUC2 表达时间来维持分生组织中起始的模式。此外，通过仔细比较野生型分生组织和沿成熟花序的叶序，我们发现这种偏差也在野生型发育过程中发生，尽管程度较小。因此，我们认为完全生长的茎中的叶序模式不仅来自分生组织的器官发生活性，而且还来自茎发育过程中随后的生长模式。
In plants, the arrangement of organs along the stem (phyllotaxy) follows a predictable pattern. Recent studies have shown that primordium position at the meristem is governed by local auxin gradients, but little is known about the subsequent events leading to the phyllotaxy along the mature stem. We show here that plants expressing a miR164-resistant CUP-SHAPED COTYLEDON2 (CUC2) gene have an abnormal phyllotactic pattern in the fully grown stem, despite the pattern of organ initiation by the meristem being normal. This implies that abnormal phyllotaxy is generated during stem growth. These plants ectopically express CUC2 in the stem, suggesting that the proper timing of CUC2 expression is required to maintain the pattern initiated in the meristem. Furthermore, by carefully comparing the phyllotaxy in the meristem and along the mature inflorescence in wild types, we show that such deviation also occurs during wild-type development, although to a smaller extent. We therefore suggest that the phyllotactic pattern in a fully grown stem results not only from the organogenetic activity of the meristem, but also from the subsequent growth pattern during stem development.