Candidacidal activity of mouse macrophages in vitro
Candidacidal activity of mouse macrophages in vitro
复制标题
小鼠巨噬细胞体外的念珠菌活性
作者：
P. Maiti;R. Kumar;L. Mohapatra
用白色念珠菌体外感染小鼠腹腔巨噬细胞，通过镜检检测细胞的吞噬活性和杀念珠菌活性。从接种bcg的动物或通过体外暴露于植物血凝素诱导的淋巴因子的正常巨噬细胞中获得的活化巨噬细胞比正常巨噬细胞表现出更高的吞噬和杀白细胞活性。然而，通过体外暴露于念珠菌诱导的淋巴因子获得的活化巨噬细胞表现出最高的吞噬和杀念珠菌活性。将免疫小鼠血清掺入培养液中也能增强正常巨噬细胞的吞噬活性和杀白细胞活性，但不能改善活化巨噬细胞的功能。这些结果表明，激活的巨噬细胞和抗体可能需要控制小鼠的念珠菌感染。
Mouse peritoneal macrophages were infected in vitro with Candida albicans, and the phagocytic and candidacidal activities were estimated by microscopic examination of Giemsa-stained cells. Activated macrophages obtained from either BCG-vaccinated animals or by in vitro exposure of normal macrophages to phytohemagglutinin-induced lymphokines exhibited higher phagocytic and candidacidal activities than did normal macrophages. However, activated macrophages obtained by in vitro exposure of macrophages to candida-induced lymphokines exhibited the highest phagocytic and candidacidal activities. The incorporation of immune mouse serum into the culture medium also enhanced the phagocytic and candidacidal activities of the normal macrophages but failed to improve the function of the activated macrophages. These results suggest that both activated macrophages and antibodies may be required for controlling candida infections in mice.