Geographic Variation in Bacterial Communities Associated With the Red Turpentine Beetle (Coleoptera: Curculionidae)
Geographic Variation in Bacterial Communities Associated With the Red Turpentine Beetle (Coleoptera: Curculionidae)
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DOI：
10.1603/en09221
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发表时间：
2010-04-01
影响因子：
1.7
通讯作者：
Raffa, Kenneth F.
中科院分区：
文献类型：
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作者：
Adams, Aaron S.;Adams, Sandye M.;Raffa, Kenneth F.
已知细菌群落在昆虫生活史中起着重要作用，但对它们在种群间的一致性或变异性知之甚少。从威斯康星州到俄勒冈州的8个种群中，对与红脂大小蠹Dendroctonus valens Le Conte相关的细菌进行了评价和比较。我们使用的变性梯度凝胶电泳，可视化细菌多样性，或个别操作分类单位（OS），从个别甲虫的文化无关的技术。单因素相似性分析用于测试站点之间的细菌群落的差异。群落剖面分析表明，每只甲虫平均含有10个OTU，甲虫的关联频率为2 - 100%。OTU序列最接近地匹配β-和γ-变形菌，并且各匹配杆菌和放线菌。几种OTU特别丰富，最值得注意的是来自100%的放线菌和来自60%的甲虫样本的两种变形菌。一些OTU与先前描述的具有已知生化能力和生态功能的细菌相似，表明D. valens可能有助于其开发低营养和高防御化合物资源的能力。不同地点的细菌群落结构存在显著差异。这些差异的强度与站点之间的距离呈正相关，尽管其他无法解释的因素也有助于变化。
Bacterial communities are known to play important roles in insect life histories, yet their consistency or variation across populations is poorly understood. Bacteria associated with the bark beetle Dendroctonus valens Le Conte from eight populations, ranging from Wisconsin to Oregon, were evaluated and compared. We used the culture-independent technique of denaturing gradient gel electrophoresis to visualize bacterial diversity, or individual operational taxonomic units (Os), from individual beetles. One-way analysis of similarities was used to test for differences of bacterial communities between sites. Analysis of community profiles showed that individual beetles on average contained 10 OTUs, with frequency of association from 2 to 100% of beetles. OTU sequences most closely matched beta- and gamma-proteobacteria, and one each matched Bacilli and Actinobacteria. Several OTUs were particularly abundant, most notably an Actinobacterium from 100% and two Proteobacteria from 60% of beetles sampled. Some OTUs were similar to previously described bacteria with known biochemical capabilities and ecological functions, suggesting that some bacterial associates of D. valens may contribute to its ability to exploit a resource low in nutrients and high in defensive compounds. There were significant differences of bacterial communities between sites. The strength of these differences was positively correlated with distance between sites, although additional unexplained factors also contribute to the variation.