Cerebellar role in fear-conditioning consolidation
Cerebellar role in fear-conditioning consolidation
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DOI：
10.1073/pnas.112660399
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发表时间：
2002-06-11
影响因子：
11.1
通讯作者：
Bucherelli, C
中科院分区：
文献类型：
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作者：
Sacchetti, B;Baldi, E;Bucherelli, C
一些小脑结构被认为参与了几种条件反射的记忆。采用行为学和神经生理学相结合的方法研究了间位核（IN）和小脑蚓部（VE）在恐惧条件化巩固中的作用。IN和VE进行完全可逆的河豚毒素（TTX）失活巩固在成年雄性Wistar大鼠，进行声学条件刺激（CS）和上下文恐惧训练。TTX注射在不同组的大鼠在收购会议后增加的时间间隔。记忆力评估为在保持测试期间测量的条件冻结持续时间，总是在立体定位TTX给药后72和96小时进行。这个时间表确保了在获取或提取阶段都不会干扰正常的小脑功能，因此任何遗忘效应都可能仅仅是由于巩固中断。我们的研究结果表明，在功能的完整性是必要的声学CS恐惧反应记忆形成96小时后收购延迟。VE功能的完整性被证明是必要的记忆形成的背景下（高达96小时后收购延迟）和声CS（高达192小时后收购延迟）的恐惧反应。目前的研究结果有助于阐明小脑在记忆巩固中的作用，并更好地定义参与恐惧记忆的神经回路。
Some cerebellar structures are known to be involved in the memorization of several conditioned responses. The role of the interpositus nucleus (IN) and the vermis (VE) in fear-conditioning consolidation was investigated by means of a combined behavioral and neurophysiological technique. The IN and VE were subjected to fully reversible tetrodotoxin (TTX) inactivation during consolidation in adult male Wistar rats that underwent acoustic conditioned stimulus (CS) and context fear training. TTX was injected in different groups of rats at increasing intervals after the acquisition session. Memory was assessed as conditioned freezing duration measured during retention testing, always performed 72 and 96 h after the stereotaxic TTX administration. This schedule ensures that there is no interference with normal cerebellar function during either the acquisition or the retrieval phase so that any amnesic effect may be due only to consolidation disruption. Our results show that IN functional integrity is necessary for acoustic CS fear response memory formation up to the 96-h after-acquisition delay. VE functional integrity was shown to be necessary for memory formation of both context (up to the 96-h after-acquisition delay) and acoustic CS (up to the 192-h after-acquisition delay) fear responses. The present findings help to elucidate the role of the cerebellum in memory consolidation and better define the neural circuits involved in fear memories.