Flow rate-controlled pipetting for microfluidics: second-generation flexible hydraulic reservoir (FHRv2)
Flow rate-controlled pipetting for microfluidics: second-generation flexible hydraulic reservoir (FHRv2)
复制标题
DOI：
10.1007/s10404-020-02402-x
复制
发表时间：
2021-01
影响因子：
2.8
通讯作者：
Atakan Atay;Alper Topuz;Büşra Sarıarslan;E. Yıldırım;J. Charmet;K. Couling;B. Çetin
中科院分区：
文献类型：
--
作者：
Atakan Atay;Alper Topuz;Büşra Sarıarslan;E. Yıldırım;J. Charmet;K. Couling;B. Çetin
微流体技术的一个关键组成部分是流体泵送机构。注射器和压力泵通常用于实验室环境;然而，它们的操作产生相当大的死体积，通常大于芯片本身的体积，导致大量宝贵样品的浪费。作为替代方案，移液允许精确的液体分配，具有零死体积;然而，它具有有限的流量控制。最近，我们推出了一种低成本的样品加载接口与零死体积命名为柔性液压水库（FHR）。在这项研究中，我们提出了第二代FHRv 2，它结合了连续泵送，零死体积和移液的多功能性。FHRv 2的性能进行了测试，对注射泵的流量范围在20和60 L/min之间。它表现出更平稳的操作和相同的瞬态时间，以达到稳定的流量，证实了为该场合开发的数学模型。重要的是，我们还证明了FHRv 2可以防止典型注射泵在分配颗粒时通常会遇到的沉积诱导的伪影。最后，我们展示了使用3D打印模具注塑成型的FHRv 2概念的制造。总的来说，我们的FHRv 2为微流体设备中的零体积液体处理提供了一种低成本和多功能的解决方案。
A critical component of microfluidic technology is the fluid pumping mechanism. Syringe and pressure pumps are typically used in the lab environment; however, their operations generate considerable dead volume that is often larger than the volume of the chip itself, leading to considerable waste of precious sample. As an alternative, pipetting allows for precise liquid dispensing with zero dead volume; however, it has a limited flow control. Recently, we have introduced a low-cost sample loading interface with zero dead-volume named flexible hydraulic reservoir (FHR). In this study, we present a second-generation FHRv2 that combines continuous pumping, zero-dead volume and the versatility of pipetting. The performance of FHRv2 is tested against a syringe pump at flow rates ranging between 20 and 60L/min. It demonstrated smoother operation and identical transient time to reach steady flow rate as confirmed by a mathematical model developed for the occasion. Importantly, we also demonstrate that the FHRv2 prevents sedimentation-induced artifacts typically encountered in typical syringe pumps when dispensing particles. Finally, we demonstrate the fabrication of the FHRv2 concept with injection molding using a 3D-printed mold. Overall, our FHRv2 offers a low-cost and versatile solution for zero-volume liquid handling in microfluidic devices.