Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex
Precise switching of DNA replication timing in the GC content transition area in the human major histocompatibility complex
复制标题
人类主要组织相容性复合体中 GC 含量转换区 DNA 复制时间的精确切换
DOI：
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复制
发表时间：
1997
影响因子：
5.3
通讯作者：
Toshimichi Ikemura
中科院分区：
文献类型：
--
作者：
T. Tenzen;Tetsushi Yamagata;Tatsuo Fukagawa;Kimihiko Sugaya;Asako Ando;H. Inoko;Takashi Gojobori;Asao Fujiyama;Katsuzumi Okumura;Toshimichi Ikemura
人类基因组由被认为与染色体带相关的长程G+C%（GC%）嵌合结构组成。我们以前报道的边界的巨型碱基大小的GC%镶嵌域之间的主要组织相容性复合体（MHC）类II和III的交界处，提出它作为一个可能的染色体带边界。在S期的DNA复制定时是已知的相关细胞遗传学与染色体带区，因此带边界已被预测为包含一个开关点的DNA复制定时。在这项研究中，以确定在S期的复制开关点的核苷酸序列水平，我们确定了精确的DNA复制时间的MHC II类和III类，集中在交界区。为了做到这一点，我们使用基于PCR的定量从同步化的人髓性白血病HL 60细胞获得的新生DNA。复制时间精确地改变在边界区域与2小时之间的差异两侧，支持预测，该区域可能是一个染色体带边界。我们假设，复制叉运动终止（暂停）或显着放缓的开关区域，其中包含密集的铝簇;多嘌呤/多嘧啶tracts;二，三，或四核苷酸重复;和中等重复频率的序列。由于开关区的新生DNA回收效率较低，我们研究了该区域是否与核支架相关，并在开关区及其周围发现了三个支架相关区域。
The human genome is composed of long-range G+C% (GC%) mosaic structures thought to be related to chromosome bands. We previously reported a boundary of megabase-sized GC% mosaic domains at the junction area between major histocompatibility complex (MHC) classes II and III, proposing it as a possible chromosome band boundary. DNA replication timing during the S phase is known to be correlated cytogenetically with chromosome band zones, and thus the band boundaries have been predicted to contain a switch point for DNA replication timing. In this study, to identify to the nucleotide sequence level the replication switch point during the S phase, we determined the precise DNA replication timing for MHC classes II and III, focusing on the junction area. To do this, we used PCR-based quantitation of nascent DNA obtained from synchronized human myeloid leukemia HL60 cells. The replication timing changed precisely in the boundary region with a 2-h difference between the two sides, supporting the prediction that this region may be a chromosome band boundary. We supposed that replication fork movement terminates (pauses) or significantly slows in the switch region, which contains dense Alu clusters; polypurine/polypyrimidine tracts; di-, tri-, or tetranucleotide repeats; and medium-reiteration-frequency sequences. Because the nascent DNA in the switch region was recovered at low efficiency, we investigated whether this region is associated with the nuclear scaffold and found three scaffold-associated regions in and around the switch region.
中国仓鼠卵巢细胞 DNA 附着蛋白的鉴定。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cress,AE;Kurath,KM
通讯作者：
Kurath,KM
DOI：
10.1073/pnas.87.7.2725
发表时间：
1990-04-01
影响因子：
11.1
作者：
GILLILAND, G;PERRIN, S;BUNN, HF
通讯作者：
BUNN, HF
真核 Z-三链体 DNA 基序对 COS-7 细胞中附加型 DNA 复制的位置和方向依赖性影响。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Brinton,BT;Caddle,MS;Heintz,NH
通讯作者：
Heintz,NH