Investigation of the cerebral hemodynamic response function in single blood vessels by functional photoacoustic microscopy
Investigation of the cerebral hemodynamic response function in single blood vessels by functional photoacoustic microscopy
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DOI：
10.1117/1.jbo.17.6.061210
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发表时间：
2012-06-01
影响因子：
3.5
通讯作者：
Li, Meng-Lin
中科院分区：
文献类型：
--
作者：
Liao, Lun-De;Lin, Chin-Teng;Li, Meng-Lin
血流动力学反应函数（HRF）的特异性在空间上由血管结构决定，在时间上由血流动力学变化的演变决定。在这里，我们使用功能性光声显微镜（fPAM），以探讨单一的脑血管大鼠左前爪刺激后。在该系统中，我们分析了总血红蛋白浓度（HbT），脑血容量（CBV）和血红蛋白氧饱和度（SO2）的HRF的时空演变。对应于不同电刺激强度和持续时间的特定脑血管的变化以36 × 65 μ m（2）的空间分辨率双侧成像。施加1、2、6和10 mA的刺激强度，持续5或15 s。我们的研究结果表明，HbT，CBV和SO2的相对功能变化不仅高度依赖于刺激的强度，而且还依赖于其持续时间。此外，刺激的持续时间对HRF的时空特征具有强烈的影响，因为较短的刺激仅在局部脉管系统（较小的小动脉）中引起响应，而较长的刺激导致更大的血管供应和引流。这项研究表明，目前的fPAM系统是可靠的研究相对脑血流动力学的变化，以及提供新的见解功能性脑血流动力学的变化在小动物的动力学。(C)2012年，美国光电仪器工程师学会（SPIE）。[ DOI：10.1117/1.JBO.17.6.061210]
The specificity of the hemodynamic response function (HRF) is determined spatially by the vascular architecture and temporally by the evolution of hemodynamic changes. Here, we used functional photoacoustic microscopy (fPAM) to investigate single cerebral blood vessels of rats after left forepaw stimulation. In this system, we analyzed the spatiotemporal evolution of the HRFs of the total hemoglobin concentration (HbT), cerebral blood volume (CBV), and hemoglobin oxygen saturation (SO2). Changes in specific cerebral vessels corresponding to various electrical stimulation intensities and durations were bilaterally imaged with 36 x 65-mu m(2) spatial resolution. Stimulation intensities of 1, 2, 6, and 10 mA were applied for periods of 5 or 15 s. Our results show that the relative functional changes in HbT, CBV, and SO2 are highly dependent not only on the intensity of the stimulation, but also on its duration. Additionally, the duration of the stimulation has a strong influence on the spatiotemporal characteristics of the HRF as shorter stimuli elicit responses only in the local vasculature (smaller arterioles), whereas longer stimuli lead to greater vascular supply and drainage. This study suggests that the current fPAM system is reliable for studying relative cerebral hemodynamic changes, as well as for offering new insights into the dynamics of functional cerebral hemodynamic changes in small animals. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [ DOI: 10.1117/1.JBO.17.6.061210]