Application of nitric oxide and carbon monoxide in a model of renal preservation
Application of nitric oxide and carbon monoxide in a model of renal preservation
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DOI：
10.1002/bjs.6174
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发表时间：
2008-08-01
影响因子：
9.6
通讯作者：
Nicholson, M. L.
中科院分区：
文献类型：
--
作者：
Hosgood, S. A.;Bagul, A.;Nicholson, M. L.
背景：一氧化氮和一氧化碳可引起血管舒张性缺血再灌注损伤。采用离体猪肾模型，评价在再灌注后温保存期间给予一氧化氮供体硝普钠（SNP）和一氧化碳释放分子（CORM） 3的效果。方法：将热缺血10 min、冷藏16 h后的肾脏分为SNP组、对照组、CORM-3组和失活CORM-3组（失活对照组）。评估肾功能和生存能力。结果：SNP和CORM-3增加了保温期间肾血流量（RBF） （P = 0.014）。再灌注后，CORM-3组RBF较对照组明显改善（P = 0.019）。与停用CORM-3组相比，CORM-3组肌酐清除率降低幅度明显小于停用CORM-3组（P = 0.021），血清肌酐水平显著降低（P = 0.029）。保温时RBF与再灌注时功能参数呈负相关(肌酐浓度：r(s) = -0.722, P < 0.001；钠排泄：r(s) = -0.912, P < 0.001)。结论：CORM-3具有良好的血管扩张作用，可改善再灌注肾功能；SNP产生了类似的影响，尽管不那么明显。
Background: Nitric oxide and carbon monoxide exert vasodilatory ischaemia-reperfusion injury. An isolated porcine kidney model was used to assess the effects of administering the nitric oxide donor sodium nitroprusside (SNP) and carbon monoxide-releasing molecule (CORM) 3 during a period of warm preservation followed by reperfusion.Methods: Kidneys were perfused under warm preservation conditions after 10 min of warm ischaemia and 16 h of cold storage in four groups: SNP, control, CORM-3 and inactive CORM-3 (inactive control). Renal function and viability were assessed.Results: SNP and CORM-3 increased renal blood flow (RBF) during warm preservation (P = 0.014). After reperfusion, RBF was significantly improved in the CORM-3 group compared with the control group (P = 0.019). The reduction in creatinine clearance was significantly less in the CORM-3 group than in the inactive CORM-3 group (P = 0.021), and serum creatinine levels were significantly lower (P = 0.029). There was a negative correlation between RBF during warm preservation and functional parameters during reperfusion (creatinine concentration: r(s) = -0.722, P < 0.001; sodium excretion: r(s) = -0.912, P < 0.001).Conclusion: The beneficial vasodilatory effects of CORM-3 during warm preservation improved renal function during reperfusion; SNP exerted similar, although less pronounced, effects.