Application of single immunoaffinity clean-up for simultaneous determination of regulated mycotoxins in cereals and nuts.
Application of single immunoaffinity clean-up for simultaneous determination of regulated mycotoxins in cereals and nuts.
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DOI：
10.1016/j.talanta.2013.09.007
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发表时间：
2013-12
期刊：
影响因子：
6.1
通讯作者：
Marta Václavíková;S. Macmahon;Kai Zhang;T. Begley
中科院分区：
文献类型：
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作者：
Marta Václavíková;S. Macmahon;Kai Zhang;T. Begley
建立了超高效液相色谱-串联质谱（UHPLC-MS/MS）同时测定12种真菌毒素（黄曲霉毒素、伏马菌素、玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇、赭曲霉毒素A、T-2和HT-2毒素）的方法。对花生、大麦和玉米早餐谷物进行了方法验证;选择这些谷物是因为它们代表了最常被真菌毒素污染的基质。该方法专为监管、工业和私人实验室快速可靠地分析真菌毒素而设计。含有本文研究的所有真菌毒素的抗体的多靶免疫亲和柱用于样品净化。方法优化主要集中在简化柱生产商推荐的提取和净化程序。这种新开发的简化程序减少了样品制备时间和用于其处理的溶剂体积。所有受管制的真菌毒素的分析都是通过新开发的UHPLC-MS/MS方法进行的，样品运行时间仅为10分钟。该方法的真实性进行了测试，分析加标和认证的参考材料，回收率范围为71%至112%的所有检查的真菌毒素。
A rapid and sensitive analytical strategy for the simultaneous determination of twelve mycotoxins (aflatoxins, fumonisins, zearalenon, deoxynivalenol, ochratoxin A, T-2 and HT-2 toxins) using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) was developed and validated. The method was validated for peanuts, barley and maize-breakfast cereals; selected as they represent the matrices most often contaminated by mycotoxins. The method is designed for fast and reliable analyses of mycotoxins in regulatory, industrial and private laboratories. Multi-target immunoaffinity columns containing antibodies for all mycotoxins studied herein were used for sample clean-up. Method optimization was predominantly focused on the simplification of extraction and clean-up procedure recommended by column producers. This newly developed and simplified procedure decreased both the sample preparation time and the solvent volumes used for their processing. The analysis of all regulated mycotoxins was conducted by a newly developed UHPLC–MS/MS method with a sample run time of only ten minutes. The method trueness was tested with analytical spikes and certified reference materials, with recoveries ranging from 71% to 112% for all of the examined mycotoxins.