Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion
Eco1 is a novel acetyltransferase that can acetylate proteins involved in cohesion
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DOI：
10.1016/s0960-9822(02)00681-4
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发表时间：
2002-02-19
期刊：
影响因子：
9.2
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Ivanov, D;Schleiffer, A;Nasmyth, K
姐妹染色单体之间的凝聚力在S期建立，并在G2期保持，直到后期分解(综述见[1-3])。在酿酒酵母中，一个由Scc1、Smc1、Smc3和Scc3蛋白组成的复合体，称为“粘附素”，调节姐妹染色单体之间的连接[4-6]。进化保守的酵母蛋白Eco1是S时期姐妹染色单体凝聚力建立所必需的，但不是在G2或M期进一步维持所需的，也不是将粘连蛋白复合体装载到DNA上[6，7]。我们用敏感的序列分析技术，包括隐马尔可夫模型结构域片段搜索，解决了乙醇组分的分子功能。我们发现了一个具有N-端C2H2锌指状结构域和类似于150个残基的C-末端结构域的双结构域结构，其中明显的乙酰辅酶A结合基序为(http://mendel.imp.univie.ac.at/SEQUENCES/ECO1/).生化试验证实，Eco1在体外具有乙酰转移酶活性。在体外，乙二醇乙酰化自身和粘附素复合体的组成部分，但不是组蛋白。因此，姐妹染色单体之间的凝聚力的建立似乎直接或间接地受到特定的乙酰转移酶的调节。
Cohesion between sister chromatids is established during S phase and maintained through G2 phase until it is resolved in anaphase (for review, see [1-3]). In Saccharomyces cerevisiae, a complex consisting of Scc1, Smc1, Smc3, and Scc3 proteins, called "cohesin," mediates the connection between sister chromatids [4-6]. The evolutionary conserved yeast protein Eco1 is required for establishment of sister chromatid cohesion during S phase but not for its further maintenance during G2 or M phases or for loading the cohesin complex onto DNA [6, 7]. We address the molecular functions of Ecol with sensitive sequence analytic techniques, including hidden Markov model domain fragment searches. We found a two-domain architecture with an N-terminal C2H2 Zn finger-like domain and an similar to150 residue C-terminal domain with an apparent acetyl coenzyme A binding motif (http://mendel.imp.univie.ac.at/SEQUENCES/ECO1/). Biochemical tests confirm that Eco1 has the acetyltransferase activity in vitro. In vitro Ecol acetylates itself and components of the cohesin complex but not histones. Thus, the establishment of cohesion between sister chromatids appears to be regulated, directly or indirectly, by a specific acetyltransferase.