Gold Nanoparticles Bearing Functional Anti-Cancer Drug and Anti-Angiogenic Agent: A "2 in 1" System with Potential Application in Cancer Therapeutics
Gold Nanoparticles Bearing Functional Anti-Cancer Drug and Anti-Angiogenic Agent: A "2 in 1" System with Potential Application in Cancer Therapeutics
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DOI：
10.1166/jbn.2005.016
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发表时间：
2005-06-01
影响因子：
2.9
通讯作者：
Mukhopadhyay, Debabrata
中科院分区：
文献类型：
--
作者：
Mukherjee, Priyabrata;Bhattacharya, Resham;Mukhopadhyay, Debabrata
血管生成是肿瘤生长和转移的必要步骤，是从原有血管形成新血管的过程。抗血管生成分子和细胞毒药物联合治疗是一种很有前途的抗癌治疗方法。然而，抗癌药物使用的根本问题是它们的半衰期短，具有广泛的全身毒性。然而，当以金结合的形式给药时，这些分子可能会增加在体内的保留，并降低全身毒性。在这项研究中，报道了一种由纳米金核心和抗癌药物吉西他滨组成的纳米复合系统，该纳米金核心含有抗血管生成分子VEGF抗体-2C3(AbVF)。利用紫外可见光谱(UV-Vis)和透射电子显微镜(TEM)对所制备的纳米复合材料进行了表征。在人脐静脉内皮细胞(HUVEC)和786-O细胞上测试了纳米复合材料中各组分的功能活性，结果表明，该纳米复合材料保留了VEGF抗体和吉西他滨的功能活性。这提供了一个独特的“2合1”系统，其中两个具有不同功能的组件已连接到单个金芯上，但单个组件的功能活性仍被保留。这些纳米复合材料可能会在癌症治疗中找到潜在的应用。
Angiogenesis, the formation of new blood vessels from a pre-existing vessel, is a necessary step for tumor growth and metastasis. Combination therapy using anti-angiogenic molecule and cytotoxic agents is a promising approach in anti-cancer therapy. However, the underlying problem in the use of anti-cancer drug is that they have short half-lives with extensive systemic toxicity. However, when delivered in a gold-conjugated form, these molecules might have increased retention in the body with reduced systemic toxicity. In this study a nano-composite system consisting of a nanogold core bearing an anti-angiogenic molecule, VEGF antibody-2C3 (AbVF), along with an anti-cancer drug, gemcitabine has been reported. The physico-chemical characterization of these nanocomposites was performed using UV-Visible (UV-Vis) spectroscopy and transmission electron microscopy (TEM). The functional activity of the individual components in the nanocomposites was tested on HUVEC (human umbilical vein endothelial cells) and 786-O cells and showed retention of functional activity of both VEGF antibody and gemcitabine. This provides a unique "2 in 1" system where 2 components with different functions have been attached onto a single gold core, yet the functional activity of the individual components are retained. These nano-composites might find potential applications in the treatment of cancer.