Distribution of nonintegrated DNA from JC papovavirus in organs of patients with progressive multifocal leukoencephalopathy.
Distribution of nonintegrated DNA from JC papovavirus in organs of patients with progressive multifocal leukoencephalopathy.
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JC 乳多空病毒非整合 DNA 在进行性多灶性白质脑病患者器官中的分布。
DOI：
10.1093/infdis/147.4.669
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Walker,DL
中科院分区：
文献类型：
--
作者：
Grinnell,BW;Padgett,BL;Walker,DL
对10例进行性多灶性白质脑病（PML）和14例血清学JC乳多空病毒阳性的非PML患者的组织进行了人多瘤病毒DNA序列的分子杂交检查。尽管荧光抗体法未鉴定出病毒蛋白，但通过杂交在9名PML患者中的7名患者的肾脏中发现了病毒DNA，每个细胞基因组当量中有0.2-10个病毒基因组拷贝，而在大脑病变区域中每个细胞中有0.6-4 × 103个拷贝。通过印迹杂交检测PML患者脑和肾中的病毒DNA，未发现整合到宿主细胞基因组中的证据。在3例PML患者中，在肝、肺、淋巴结和脾中也发现了病毒DNA。这些患者中有两名是儿童，患有广泛传播的JC病毒。在没有PML的患者的组织中，没有发现JC病毒感染的证据，但在14个肾脏中的两个检测到BK乳多空病毒DNA。
Tissues from 10 patients with progressive multifocal leukoencephalopathy (PML) and 14 patients without PML who were serologically positive for JC papovavirus were examined by molecular hybridization for human polyomavirus DNA sequences. Although viral proteins were not identified by fluorescent antibody methods, viral DNA was found in the kidneys of seven of nine patients with PML by hybridization, at 0.2–10 viral genome copies per cell genome equivalent, compared with 0.6–4 × 103copies per cell in diseased areas of the brain. Examination of viral DNA from brains and kidneys of patients with PML by blot hybridization yielded no evidence of integration into the host cell genome. In three of the patients with PML, viral DNA was also found in liver, lung, lymph node, and spleen. Two of these patients, with widely disseminated JC virus, were children. In tissues from patients without PML, no evidence of JC virus infection was found, but BK papovavirus DNA was detected in two of 14 kidneys tested.