Human epithelial cells increase their rigidity with ageing in vitro:: direct measurements
Human epithelial cells increase their rigidity with ageing in vitro:: direct measurements
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DOI：
10.1088/0031-9155/50/1/007
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发表时间：
2005-01-07
影响因子：
3.5
通讯作者：
Sokolov, I
中科院分区：
文献类型：
--
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
随着年龄的增长，上皮组织的弹性下降导致许多人类疾病。这种变化以前归因于细胞外基质蛋白的交联增加。在这里，我们表明，在体外老化过程中，单个人类上皮细胞也变得更加坚硬。使用原子力显微镜（AFM），我们发现活细胞的杨氏模量在老年细胞与年轻细胞中持续增加2至4倍。直接可视化的细胞骨架使用一种新的方法，涉及原子力显微镜表明，增加刚度的老化细胞是由于更高密度的细胞骨架纤维。我们的研究结果确定了一个独特的机制，可能有助于与年龄相关的弹性损失上皮组织。
The decrease in elasticity of epithelial tissues with ageing contributes to many human diseases. This change was previously attributed to increased crosslinking of extracellular matrix proteins. Here we show that individual human epithelial cells also become significantly more rigid during ageing in vitro. Using atomic force microscopy (AFM), we found that the Young's modulus of viable cells was consistently increased two- to four-fold in older versus younger cells. Direct visualization of the cytoskeleton using a novel method involving the AFM suggested that increased rigidity of ageing cells was due to a higher density of cytoskeletal fibres. Our results identify a unique mechanism that might contribute to the age-related loss of elasticity in epithelial tissues.