Antibody-induced anemia in fetal sheep: model for hemolytic disease of the fetus and newborn.
Antibody-induced anemia in fetal sheep: model for hemolytic disease of the fetus and newborn.
复制标题
抗体诱导的胎羊贫血：胎儿和新生儿溶血病模型。
DOI：
10.1016/s1071-5576(01)00107-1
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Brace,RA
中科院分区：
文献类型：
--
作者：
Wolf,RB;MoiseJr,KJ;Brace,RA
ObjectiveOur purpose was to produce a condition analogous a alloimmune hemolytic disease of the fetus and newborn by infusing antierythrocyte antibodies in fetal sheep.MethodsAntierythrocyte antibodies were infused intravascularly into late-gestation ovine fetuses over a 10-day period. Fetal blood was sampled dialy for complete blood cell counts, blood gases, iron, erythropietin (EPO), and electrolyte concentrations. Red cell mass (RCM) and blood volume were determined every other day using indicator dilution techniques. Results were compared with eight similarly aged control animals. Statistical analysis included Student t test, three-factor analysis of variance, and least squares regression.ResultsThe hematocrit in seven fetal sheep receiving antibody infusion declined significantly by 10.3 ± 1.7%, whereas it increased in control animals 2.3 ± 0.6% (P< .001). RCM was reduced by 18.9 ± 3.2% over the 10-day protocol while increasing 34.1 ± 4.2% in control animals, representing more than a 50% difference in RCM (P< .001). Fetal EPO was significantly increased with lower hematocrit and lower Po2(P< .001). As fetal hematocrit declined below 25%, lactate and reticulocytes also increased (P< .001). Plasma iron concentration was not significantly altered (P= .47).ConclusionsThe chronically catheterized fetal sheep is a viable model for studying immunologically induced fetal anemia as hematocrit can be tirated and the fall in RCM and hematocrit are associated with fetal hypoxia and elevated EPO as occurs in the anemic human fetus. Furthermore, there appears to be a threshold degree of anemia required to elicit responses as the fetal EPO, PO2, and lactate appeared unresponsive until hematocrit fell below 25%.
