Enhanced Performance of Rhizopus oryzae Lipase Immobilized on Hydrophobic Carriers and Its Application in Biorefinery of Rapeseed Oil Deodorizer Distillate
Enhanced Performance of Rhizopus oryzae Lipase Immobilized on Hydrophobic Carriers and Its Application in Biorefinery of Rapeseed Oil Deodorizer Distillate
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DOI：
10.1007/s12155-014-9415-y
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发表时间：
2014-02
影响因子：
3.6
通讯作者：
Feng Su;Guanlin Li;Houjin Zhang;Yunjun Yan
中科院分区：
文献类型：
--
作者：
Feng Su;Guanlin Li;Houjin Zhang;Yunjun Yan
In this study, hydrophobic macroporous resin NKA was employed as matrix for immobilization of freeRhizopus oryzaelipase (ROL). The performance of the immobilized ROL was significantly enhanced. The recovery activity was up to 1,293.78 % and the specific activity increased to 152,914 U/g-protein, which was 46-fold higher than that of the free lipase. Moreover, the immobilized lipase showed higher thermostability and better pH-resistance than its free counterpart. Additionally, three different nonaqueous modification strategies (including bioimprinting, lecithin coating, and lyophilization protection) were further utilized to improve the performance of the immobilized lipase. The corresponding enhancements were 33.68 %, 31.98 %, and 99.86 %. When these modifications were combined together, the activity improved 209.51 %. In order to confirm its practical application, the modified ROL was used to biorefine rapeseed oil deodorizer distillate (RODD) for biodiesel production. The highest conversion yield reached 98.23 %, much close to that (97.46 %) of Novozym 435. The results suggest that the prepared lipase in this study is a promising biocatalyst with high stability, efficiency and operational reusability.
