Two new lncRNAs regulate the key immune factor NOD1 and TRAF5 in chicken lymphocyte
Two new lncRNAs regulate the key immune factor NOD1 and TRAF5 in chicken lymphocyte
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两种新的lncRNA调控鸡淋巴细胞关键免疫因子NOD1和TRAF5
DOI：
10.1016/s2095-3119(19)62722-x
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发表时间：
2019-11-01
影响因子：
4.8
通讯作者：
Chen Guo-hong
中科院分区：
文献类型：
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作者：
Bi Yu-lin;Yuan Xiao-ya;Chen Guo-hong
禽网状内皮组织增生病病毒（REV）可引起鸡免疫器官萎缩和免疫抑制，但其免疫应答的分子机制尚不清楚。目前，RNA-seq用于分析鸡外周血淋巴细胞对REV的免疫应答调节。总体而言，筛选了134个在有REV感染或没有体外感染的细胞之间差异表达的长非编码RNA（lncRNA）。基于差异表达的蛋白质编码基因，丰富了与免疫调节相关的核苷酸结合寡聚化结构域（NOD）样受体通路。两个lncRNA（L11530和L09863）被预测分别靶向NOD 1和肿瘤坏死因子受体相关因子5（TRAF 5）基因，这两个基因以顺式调节方式参与NOD样受体途径。体外实验结果显示，与对照组相比，
Reticuloendotheliosis virus (REV) causes the atrophy of immune organs and immuno-suppression in chickens, but the underlying molecular mechanism of the immune response after infection by REV is not well understood. Presently, the RNA-seq was used to analyze the regulation of immune response to REV in chicken lymphocytes from peripheral blood. Overall, 134 differentially expressed long non-coding RNAs (lncRNAs) between cells with REV infection or without in vitro were screened. Based on the differentially expressed protein-coding genes, the nucleotide-binding oligomerization domain (NOD)-like receptor pathway related to immune regulation was enriched. Two lncRNAs (L11530 and L09863) were predicted to target the NOD1 and tumor necrosis factor receptor-associated factor 5 (TRAF5) gene, respectively, which are involved in the NOD-like receptor pathway with cis-regulation way. The in vitro results revealed the significantly up-regulated (P