Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile
Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile
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DOI：
10.1016/j.tvjl.2018.12.010
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发表时间：
2019-02-01
影响因子：
2.2
通讯作者：
van Weeren, P. R.
中科院分区：
文献类型：
--
作者：
Boere, J.;van de Lest, C. H. A.;van Weeren, P. R.
细胞外囊泡（EV）的浓度，特征和功能在马滑液（SF）在正常的生长和发育以前没有研究。通过差速离心法（10，000 g和200，000 g）从3匹健康马驹和2匹成年马的SF中分离EV;通过蔗糖密度梯度浮选法纯化EV，并通过高分辨率流式细胞术（FCM）、浮力密度和蛋白质印迹分析EV。此外，在10匹马驹和6匹成年马的SF中进行了硫酸化糖胺聚糖（GAG）、基质金属蛋白酶（MMP）、C-末端交联端肽II型胶原（CTX-II）、胶原酶裂解的新肽II型胶原（C2C）的重复生物标志物分析。与定量EV特征相反，幼年关节SF中的生物标志物特征与成年动物SF中的生物标志物特征显著不同。然而，在高分辨率FCM散点图中存在质的差异。未来的深入功能分析可能会揭示SF中青少年和成熟EV之间的差异。(C)2018由Elsevier Ltd.出版
Extracellular vesicle (EV) concentration, characteristics and function in equine synovial fluid (SF) during normal growth and development has not previously been studied. Isolation of EVs was performed in SF from three healthy foals and two adult horses by differential ultracentrifugation (10,000g and 200,000g); EVs were purified by sucrose density gradient floatation and analysed by high-resolution flow cytometry (FCM), buoyant density and western blotting. Additionally, repeated biomarker analysis of sulphated glycosaminoglycans (GAG), matrix metalloproteinase (MMP), C-terminal crosslinked telopeptide type II collagen (CTX-II), collagenase cleaved neopeptide type II collagen (C2C) was performed in SF from 10 foals and six adult horses. In contrast with the quantitative EV profile, the biomarker profile in SF from juvenile joints was substantially different from that in SF from adult animals. However, there were qualitative differences in the high-resolution FCM scatter plots. Future in-depth functional analyses may reveal differences between juvenile and mature EVs in SF. (C) 2018 Published by Elsevier Ltd.