Control of yeast alpha-specific genes: evidence for two blocks to expression in MATa/MAT alpha diploids.
Control of yeast alpha-specific genes: evidence for two blocks to expression in MATa/MAT alpha diploids.
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酵母 α 特异性基因的控制：MATa/MAT α 二倍体中表达的两个阻断的证据。
DOI：
10.1073/pnas.82.17.5855
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发表时间：
1985
影响因子：
11.1
通讯作者：
Bender,A
中科院分区：
文献类型：
--
作者：
Ammerer,G;SpragueJr,GF;Bender,A
在酵母α细胞中，由交配型基因座的MAT α 1基因编码的产物是至少两种基因STE 3和MF α 1转录所需的。为了了解在α和α/α细胞中缺乏MAT α 1产物是否足以解释在这些细胞中不能表达STE 3和MF α 1，我们通过一种杂合基因提供了MAT α 1产物，该杂合基因绕过了MAT α 1基因的正常调节。我们通过RNA印迹分析发现，MAT α 1蛋白的提供允许α细胞中而不是α/α细胞中产生STE 3和MF α 1 mRNA。这些数据表明，即使存在MAT α 1产物，也存在阻止α/α细胞中α特异性基因表达的额外调节机制。这种调控机制似乎控制STE 3和MF α 1的表达在转录水平，而不是在转录后水平，因为我们表明，MF α 1 mRNA提供的组成型启动子翻译和加工在a/α细胞产生功能性α因子信息素。这一结果进一步表明，α/α细胞拥有信息素成熟所必需的所有机制，尽管这些细胞通常不进行这些反应。
In yeast alpha cells, the product encoded by the MAT alpha 1 gene of the mating-type locus is required for transcription of at least two genes, STE3 and MF alpha 1. To learn whether the lack of the MAT alpha 1 product in a and a/alpha cells is sufficient to explain the failure to express STE3 and MF alpha 1 in these cells, we have provided MAT alpha 1 product via a hybrid gene that circumvents the normal regulation of the MAT alpha 1 gene. We find by RNA blot analysis that provision of MAT alpha 1 protein permits production of STE3 and MF alpha 1 mRNA in a cells but not in a/alpha cells. These data suggest the existence of an additional regulatory mechanism that prevents expression of alpha-specific genes in a/alpha cells, even when MAT alpha 1 product is present. This regulatory mechanism appears to control expression of STE3 and MF alpha 1 at the transcriptional level rather than at the posttranscriptional level, because we show that MF alpha 1 mRNA supplied from a constitutive promoter is translated and processed in a/alpha cells to yield functional alpha-factor pheromone. This result shows further that a/alpha cells possess all the machinery necessary for pheromone maturation, even though these cells do not normally carry out these reactions.