Analyses of monoclonal antibodies reacting with porcine CD5:: results from the Second International Swine CD Workshop
Analyses of monoclonal antibodies reacting with porcine CD5:: results from the Second International Swine CD Workshop
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DOI：
10.1016/s0165-2427(97)00103-7
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发表时间：
1998-01-30
影响因子：
1.8
通讯作者：
Saalmüller, A
中科院分区：
文献类型：
--
作者：
Pescovitz, MD;Book, BK;Saalmüller, A
在t细胞组分析的57个单克隆抗体中，有3个单克隆抗体属于T13簇，包括CD5a标准b53b7 （No. 174）。两种新单克隆抗体1H6/8 （No. 058）和BB6-9G12 （No. 166）均可析出55和60 kDa蛋白，与标准分子量相似。不同类型细胞的染色模式相似。两种新抗体均抑制CD5a参考单克隆抗体b53b7与外周淋巴细胞的结合。因此，这些单克隆抗体都与CD5a表位发生反应，使抗猪CD5单克隆抗体的数量达到8个，它们似乎都识别相同的表位。(C) 1998爱思唯尔科学有限公司
Among the 57 monoclonal antibodies analyzed within the T-cell group, three mAbs fell within cluster T13 including the CD5a standard b53b7 (No. 174). The two new mAbs 1H6/8 (No. 058) and BB6-9G12 (No, 166) both precipitated 55 and 60 kDa proteins that were of similar molecular weights as the standard. Staining patterns on the various cell types were similar. Both new antibodies inhibited the binding of the CD5a reference mAbs b53b7 to peripheral lymphocytes. These mAbs, therefore both react with the CD5a epitope bringing the number of anti-porcine CD5 mAbs to eight, all of which appear to recognize the same epitope. (C) 1998 Elsevier Science B.V.