D2 DOPAMINE RECEPTOR GENE-EXPRESSION IN THE RAT STRIATUM DURING ONTOGENY - AN INSITU HYBRIDIZATION STUDY
D2 DOPAMINE RECEPTOR GENE-EXPRESSION IN THE RAT STRIATUM DURING ONTOGENY - AN INSITU HYBRIDIZATION STUDY
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DOI：
10.1016/0165-3806(91)90157-e
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发表时间：
1991-05-20
期刊：
影响因子：
--
通讯作者：
BLOCH, B
中科院分区：
文献类型：
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作者：
GUENNOUN, R;BLOCH, B
采用原位杂交技术研究了D2多巴胺受体（D2R）基因在大鼠纹状体中的表达。用s -35标记的寡核苷酸探针检测D2R mRNA，一个与D2R mRNA的两个异构体（D2（415）和D2(444)）等杂交，另一个与大异构体（D2(444)）特异性杂交。在妊娠第14天，首次在纹状体原基中检测到D2R mRNA，探针对这两种异构体不敏感，并且探针对D2(444) mRNA具有特异性。第16天，纹状体外侧部分和侧脑室内侧的生发室区均有D2R mRNA表达。第18天，在尾壳核、伏隔核、嗅结节和侧脑室室管膜下区神经元中检测到D2R mRNA。显微放射自显像分析表明，标记细胞在出生前具有神经母细胞和未成熟的特征。出生后，标记细胞的地形和外观与成年动物观察到的相似。D2R mRNA存在于尾壳核、伏隔核和嗅结节神经元中。在尾核-壳核中，有一个外侧-内侧的标记梯度。从出生后第2天起，D2R基因在两种纹状体细胞中表达，小神经元可能是脑啡能细胞，大神经元具有突出的细胞质，最可能是胆碱能细胞。D2R mRNA与碘硫脲在尾壳核的结合位点同时出现，比伏隔核和嗅结节的结合位点早2天出现。这些结果表明，在个体发育过程中，大鼠纹状体神经元中的D2R基因表达具有与成人相似或相同的特征，特别是D2(444) mRNA亚型的存在。
D2 dopamine receptor (D2R) gene expression in the rat striatum was studied by in situ hybridization throughout the pre- and the postnatal period from gestational day 12 to postnatal day 8. D2R mRNA was detected with S-35-labelled oligonucleotide probes, one that hybridized equally to the two isoforms of the D2R mRNA (D2(415) and D2(444)) and the other that hybridized specifically to the large isoform (D2(444)). D2R mRNA was first detected in the striatal primordium at day 14 of gestation with the probe that recognizes indifferently the two isoforms and with the probe specific for the D2(444) mRNA. At day 16, D2R mRNA was present in the lateral part of the striatum and in the germinal ventricular zone lining the lateral ventricle. At day 18, D2R mRNA was found in neurons of the caudate-putamen, the nucleus accumbens, the olfactory tubercle and the subependymal zone lining the lateral ventricle. The microautoradiographic analysis demonstrated that the labelled cells have a neuroblastic and immature aspect before birth. After birth the topography and aspect of labelled cells was similar to the one observed in the adult animals. D2R mRNA was present in neurons of the caudate-putamen, the nucleus accumbens and the olfactory tubercle. In the caudate-putamen there was a latero-medial gradient of labelling. From postnatal day 2 onward the D2R gene was expressed in two striatal cell types, small neurons probably enkephalinergic, and large-sized neurons with prominent cytoplasm, most probably cholinergic. D2R mRNA appeared at the same time as the binding sites for iodosulpiride in the caudate-putamen and two days before the binding sites in the nucleus accumbens and olfactory tubercle. These results demonstrate D2R gene expression in the rat striatal neurons during ontogeny with features similar or identical to the ones observed in the adult especially the presence of the D2(444) mRNA isoform.