Production of Skatole and para-Cresol by a Rumen Lactobacillus sp
Production of Skatole and para-Cresol by a Rumen Lactobacillus sp
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DOI：
10.1128/aem.41.1.71-76.1981
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发表时间：
1981-01
影响因子：
4.4
通讯作者：
M. Yokoyama;J. Carlson
中科院分区：
文献类型：
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作者：
M. Yokoyama;J. Carlson
本研究的目的是检查底物特异性的几个瘤胃菌株的乳杆菌属。以前被证明产生粪臭素（3-甲基吲哚）的吲哚乙酸的脱羧。总共测试了13种化合物的脱羧酶活性。乳杆菌属菌株通过对羟基苯乙酸的脱羧产生对甲酚（4-甲基苯酚），但不从相应的羟基苯乙酸异构体产生邻甲酚或间甲酚。这些菌株还将5-羟基吲哚乙酸脱羧为5-羟基粪臭素，并将3，4-二羟基苯乙酸脱羧为甲基儿茶酚。当吲哚乙酸和对羟基苯乙酸以等摩尔浓度组合时，以0.5：1的比例产生粪臭素和对甲酚。吲哚乙酸和对羟基苯乙酸的竞争研究表明，两种不同的脱羧酶参与这些菌株的粪臭素和对甲酚的生产。这是首次证明单一细菌同时产生粪臭素和对甲酚。
The objective of this study was to examine the substrate specificity of several ruminal strains of a Lactobacillus sp. which previously was shown to produce skatole (3-methylindole) by the decarboxylation of indoleacetic acid. A total of 13 compounds were tested for decarboxylase activity. The Lactobacillus strains produced p-cresol (4-methylphenol) by the decarboxylation of p-hydroxyphenylacetic acid, but did not produce either o-cresol or m-cresol from the corresponding hydroxyphenylacetic acid isomers. These strains also decarboxylated 5-hydroxyindoleacetic acid to 5-hydroxyskatole and 3,4-dihydroxyphenylacetic acid to methylcatechol. Skatole and p-cresol were produced in a 0.5:1 ratio, when indoleacetic acid and p-hydroxyphenylacetic acid were combined in equimolar concentrations. Competition studies with indoleacetic acid and p-hydroxyphenylacetic acid suggested that two different decarboxylating enzymes are involved in the production of skatole and p-cresol by these strains. This is the first demonstration of both skatole production and p-cresol production by a single bacterium.