S-antigen and glial fibrillary acidic protein immunoreactivity in the in situ pineal gland of hamster and gerbil and in pineal grafts: developmental expression of pinealocyte and glial markers.
S-antigen and glial fibrillary acidic protein immunoreactivity in the in situ pineal gland of hamster and gerbil and in pineal grafts: developmental expression of pinealocyte and glial markers.
复制标题
仓鼠和沙鼠原位松果体以及松果体移植物中的 S 抗原和神经胶质纤维酸性蛋白免疫反应性：松果体细胞和神经胶质标记物的发育表达。
DOI：
10.1002/aja.1001920415
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Welsh,MG
中科院分区：
文献类型：
--
作者：
Li,K;Welsh,MG
采用亲和素-生物素-过氧化物酶免疫组化技术研究了金仓鼠和沙鼠出生后原位松果腺中S - Ag和GFAP的免疫反应性。S - Ag在出生后第一天（P1）就存在于沙鼠松果体中，而直到P6才出现在仓鼠松果体中。GFAP免疫反应星形细胞首次在仓鼠松果体（P7）和沙鼠松果体（P10）中观察到。从7周龄到3周龄，仓鼠和沙鼠松果体中S - Ag免疫反应性松果体细胞和GFAP免疫反应性星形胶质细胞的数量随着年龄的增加而增加。4周时，在仓鼠和沙鼠的松果腺中观察到强烈的S - Ag和GFAP免疫反应性。GFAP免疫反应星形胶质细胞均匀分布在沙鼠松果体浅层，但它们更常位于仓鼠松果体浅层的周围区域。在松果体移植方面，将新生（3-5日龄）雄性仓鼠的松果体移植到成年雄性仓鼠的第三脑室（漏斗隐窝或后第三脑室）或肾包膜下方。移植后1周内，松果体出现S‐Ag免疫反应性。到4周时，松果体移植物表现出强烈的S - Ag免疫反应性，这种反应一直维持到移植后至少12周。脑室松果体移植物中胶质细胞成熟的时间进程一般与4周前仓鼠原位松果体平行。然而，到12周时，与原位松果体相比，移植松果体中有更多的星形胶质细胞分化并产生GFAP免疫反应性。这些研究描述了S - Ag和GFAP免疫反应性在原位松果体和新生儿松果体移植中出生后的发展。
Postnatal development of S‐Ag and GFAP immunoreactivity in the in situ pineal glands of golden hamsters and gerbils was examined using the avidin‐biotin‐peroxidase immunohistochemical technique. S‐Ag was present in the gerbil pineal gland on the first postnatal day (P1), whereas it did not appear in the hamster pineal until P6. GFAP‐immunoreactive astrocytes were first observed in the hamster pineal gland on P7 and in the gerbil pineal gland on P10. The number of S‐Ag‐immunoreactive pinealocytes and GFAP‐immunoreactive astrocytes in the pineal glands of hamsters and gerbils increased with increasing age from P7 to 3 weeks. By 4 weeks, strong S‐Ag and GFAP immunoreactivity was observed in both hamster and gerbil pineal glands. GFAP‐immunoreactive stellate astrocytes were distributed evenly throughout the gerbil superficial pineal gland, but they were more often located in the peripheral region of the hamster superficial pineal. For the pineal grafts, pineal glands from neonatal (3–5 day old) hamsters were transplanted into the third cerebral ventricle (infundibular recess or posterior third ventricle) or beneath the renal capsule of adult male hamsters. S‐Ag immunoreactivity appeared in the pineal grafts within 1 week following transplantation. By 4 weeks the pineal grafts showed strong S‐Ag immunoreactivity which was maintained until at least 12 weeks after transplantation. The time course of glial cell maturation in the cerebroventricular pineal grafts is generally parallel to the hamster pineal gland in situ before 4 weeks. By 12 weeks, however, more astrocytes differentiated and developed GFAP‐immunoreactivity in the pineal grafts than in the in situ pineals. These studies have described the postnatal development of S‐Ag and GFAP immunoreactivity in in situ pineal glands and in neonatal pineal grafts.