HRT1, HRT2, and HRT3:: A new subclass of bHLH transcription factors marking specific cardiac, somitic, and pharyngeal arch segments
HRT1, HRT2, and HRT3:: A new subclass of bHLH transcription factors marking specific cardiac, somitic, and pharyngeal arch segments
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DOI：
10.1006/dbio.1999.9454
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发表时间：
1999-12-01
影响因子：
2.7
通讯作者：
Srivastava, D
中科院分区：
文献类型：
--
作者：
Nakagawa, O;Nakagawa, M;Srivastava, D
碱性螺旋-环-螺旋（bHLH）转录因子的Hairy/Enhancer of Split家族的成员在脊椎动物和果蝇胚胎中受Notch信号通路调节，并控制细胞命运和基因表达的清晰边界的建立。在这里，我们描述了一个新的亚类bHLH蛋白，HRT 1（毛相关转录因子1），HRT 2，和HRT 3，共享高同源性的毛蛋白家族，但有特点，是不同于那些毛和其他bHLH蛋白。每个HRT基因在许多器官内的不同细胞类型中表达，特别是在那些沿沿着前后轴排列的细胞中。HRT 1和HRT 2分别在心房和心室前体细胞中表达，也在心脏流出道和主动脉弓动脉中表达。HRT 1和HRT 2转录本也检测到前体的咽弓，随后在咽裂。在闪米特前体中，HRT 1和HRT 3在前体中胚层中表现出动态表达，反映了Notch-Delta信号通路的其他组分的表达。HRT基因在上皮-间充质相互作用的其他部位表达，包括发育中的肾脏、脑、肢芽和脉管系统。这种新的bHLH蛋白亚家族的独特和互补的表达模式表明，在心脏和咽弓等器官的节段性模式中，Hairy相关通路具有以前未被认识到的作用。(C)北京：科学出版社.
Members of the Hairy/Enhancer of Split family of basic helix-loop-helix (bHLH) transcription factors are regulated by the Notch signaling pathway in vertebrate and Drosophila embryos and control cell fates and establishment of sharp boundaries of gene expression. Here, we describe a new subclass of bHLH proteins, HRT1 (Hairy related transcription factor 1), HRT2, and HRT3, that share high homology with the Hairy family of proteins yet have characteristics that are distinct from those of Hairy and other bHLH proteins. Each HRT gene was expressed in distinct cell types within numerous organs, particularly in those patterned along the anterior-posterior axis. HRT1 and HRT2 were expressed in atrial and ventricular precursors, respectively, and were also expressed in the cardiac outflow tract and aortic arch arteries. HRT1 and HRT2 transcripts were also detected in precursors of the pharyngeal arches and subsequently in the pharyngeal clefts. Within semitic precursors, HRT1 and HRT3 exhibited dynamic expression in the presomitic mesoderm, mirroring the expression of other components of Notch-Delta signaling pathways. The HRT genes were expressed in other sites of epithelial-mesenchymal interactions, including the developing kidneys, brain, limb buds, and vasculature. The unique and complementary expression patterns of this novel subfamily of bHLH proteins suggest a previously unrecognized role for Hairy-related pathways in segmental patterning of the heart and pharyngeal arches, among other organs. (C) 1999 Academic Press.