Metabolomic profiling of oxalate-degrading probiotic Lactobacillus acidophilus and Lactobacillus gasseri
Metabolomic profiling of oxalate-degrading probiotic Lactobacillus acidophilus and Lactobacillus gasseri
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DOI：
10.1371/journal.pone.0222393
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发表时间：
2019-09-23
期刊：
影响因子：
3.7
通讯作者：
Garrett, Timothy J.
中科院分区：
文献类型：
--
作者：
Chamberlain, Casey A.;Hatch, Marguerite;Garrett, Timothy J.
草酸盐是许多植物性食物中普遍存在的化合物，通过肠道吸收，并在肾脏中与钙沉淀形成结石。超过80%的诊断肾结石被发现是草酸钙。形成这些结石的人通常会经历高复发率，尽管经过数十年的专门研究，但治疗方案仍然有限。最近，肠道微生物组已成为新疗法的新焦点。研究表明，选择的乳酸杆菌物种，现代益生菌补充剂中最常见的属，可以在体外降解草酸盐，甚至减少原发性高尿酸症动物模型中的尿草酸盐。虽然乳酸杆菌益生菌的健康益处在物种之间存在显着差异，但有证据支持它们作为草酸盐疾病益生菌的潜在用途。确定乳酸杆菌的独特代谢特性对于确定这些细菌如何与宿主肠道相互作用并影响整体健康至关重要。我们解决了这一需要的特点和比较的代谢组和脂质体的脂肪酸降解嗜酸乳杆菌和加氏乳杆菌使用超高效液相色谱-高分辨率质谱。我们报告了这些乳杆菌属物种的代谢谱中的许多种属特异性差异，并讨论了潜在的益生菌相关性及其差异表达所导致的功能。还描述了我们对嗜酸乳杆菌和加氏乳杆菌的草酸盐降解能力的验证，即使在其他优选碳源的存在下，通过液体闪烁计数测量体外C-14-草酸盐消耗。
Oxalate, a ubiquitous compound in many plant-based foods, is absorbed through the intestine and precipitates with calcium in the kidneys to form stones. Over 80% of diagnosed kidney stones are found to be calcium oxalate. People who form these stones often experience a high rate of recurrence and treatment options remain limited despite decades of dedicated research. Recently, the intestinal microbiome has become a new focus for novel therapies. Studies have shown that select species of Lactobacillus, the most commonly included genus in modern probiotic supplements, can degrade oxalate in vitro and even decrease urinary oxalate in animal models of Primary Hyperoxaluria. Although the purported health benefits of Lactobacillus probiotics vary significantly between species, there is supporting evidence for their potential use as probiotics for oxalate diseases. Defining the unique metabolic properties of Lactobacillus is essential to define how these bacteria interact with the host intestine and influence overall health. We addressed this need by characterizing and comparing the metabolome and lipidome of the oxalate-degrading Lactobacillus acidophilus and Lactobacillus gasseri using ultra-high-performance liquid chromatography-high resolution mass spectrometry. We report many species-specific differences in the metabolic profiles of these Lactobacillus species and discuss potential probiotic relevance and function resulting from their differential expression. Also described is our validation of the oxalate-degrading ability of Lactobacillus acidophilus and Lactobacillus gasseri, even in the presence of other preferred carbon sources, measuring in vitro C-14-oxalate consumption via liquid scintillation counting.