Isolation of Streptomyces sp. Producing Glucose-tolerant δ-Glucosidases and Properties of the Enzymes
Isolation of Streptomyces sp. Producing Glucose-tolerant δ-Glucosidases and Properties of the Enzymes
复制标题
产生耐葡萄糖 δ-葡萄糖苷酶的链霉菌的分离和酶的性质
DOI：
10.1271/bbb1961.55.979
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Kazuhiko Yamada
中科院分区：
文献类型：
--
作者：
H. Ozaki;Kazuhiko Yamada
Stveptomyces sp. No. 35从国内土壤中分离得到，分类上与S. thermodiastaticus相近。β-葡萄糖苷酶仅在细胞中有活性。用纤维素二糖诱导酶合成。超声提取的细胞重复DEAE-Toyopearl层析显示F1、F2和F3三个活性峰，以NaCl浓度梯度按此顺序以50:50:1的相对量洗脱。酶被纯化到均匀性。凝胶过滤F1、F2和F3的分子量分别为100,000、105,000和130,000，十二烷基硫酸钠-聚丙烯酰胺-凝胶电泳的分子量分别为52,000、50,000和52,000。F1和F2对PNP-β-葡萄糖苷和纤维素二糖的水解速率几乎相同，而F3对前者的水解速率稍低。葡萄糖抑制F1和F2，使其Kis分别为56和40 mm。另一方面，F3被0.1 m葡萄糖激活2倍，被1 m葡萄糖抑制50%。木糖也同样激活了F3。所有酶均含有β-半乳糖苷。
Stveptomyces sp. No. 35, taxonomically closed to S. thermodiastaticus, was isolated from domestic soil. It showed β-glucosidase activity only in the cells. The enzyme synthesis was induced by cellobiose. Repeated DEAE-Toyopearl chromatography of a sonic extract of the cells showed three activity peaks, F1, F2, and F3, which were eluted in the relative amount of 50 :50:1 in this order by an NaCl concentration gradient. The enzymes were purified to homogeneity. Molecular weights of F1 F2, and F3 were 100,000, 105,000, and 130,000 by gel filtration, and 52,000, 50,000, and 52,000, respectively, by sodium dodecyl sulfate-polyacrylamide-gel electrophesis. F1 and F2 hydrolyzed PNP-β-glucoside and cellobiose nearly at the same rate, while F3 hydrolyzed the former slightly. F1 and F2 were inhibited by glucose, giving Kis of 56 and 40 mm, respectively. On the other hand, F3 was activated twofold by 0.1 m glucose and inhibited 50% by 1 m glucose. Xylose also similarly activated F3. All the enzymes had β-galactosida...