Modulation of lipopolysaccharide-induced proinflammatory cytokine production in vitro and in vivo by the herbal constituents apigenin (chamomile), ginsenoside Rb1 (ginseng) and parthenolide (feverfew)
Modulation of lipopolysaccharide-induced proinflammatory cytokine production in vitro and in vivo by the herbal constituents apigenin (chamomile), ginsenoside Rb1 (ginseng) and parthenolide (feverfew)
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DOI：
10.1016/s0278-6915(03)00146-7
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发表时间：
2003-10-01
影响因子：
4.3
通讯作者：
Pestka, JJ
中科院分区：
文献类型：
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作者：
Smolinski, AT;Pestka, JJ
膳食补充剂不像传统药物那样需要经过同样的上市前批准，因此这些产品的真正功效和安全性尚不清楚。本研究的目的是评估三种草药成分芹菜素（洋甘菊）、人参皂苷 Rb-1（人参）和小白菊内酯（小白菊）对脂多糖 (LPS) 诱导的促炎细胞因子产生的潜在抗炎特性，并确定细胞培养的影响是否可以预测完整动物模型的结果。用刺激性 LPS 和草药成分处理小鼠巨噬细胞和小鼠，并通过 ELISA 分别评估所得培养物上清液和血清中的白细胞介素 (IL)-6 和肿瘤坏死因子 (TNF)-α。所有三种成分均抑制培养物中 LPS 诱导的 IL-6 和/或 TNF-α 的产生。在小鼠中观察到这两种细胞因子的抑制，但没有表现出与细胞培养数据相同的抑制模式。结果表明，所有三种成分都具有抗炎特性，但细胞培养数据只能用于估计对动物的潜在影响，并且必须使用适当的动物模型进行确认。 (C) 2003 Elsevier Ltd. 保留所有权利。
Dietary supplements are not subject to the same pre-market approval as conventional drugs, thus the true efficacy and, in cases, safety of these products is not known. The objective of this study was to evaluate the potential anti-inflammatory properties of three herbal constituents, apigenin (chamomile), ginsenoside Rb-1, (ginseng) and parthenolide (feverfew) on lipopolysaccaharide (LPS)-induced proinflammatory cytokine production, and to determine if effects in cell culture could predict results in an intact animal model. Murine macrophage cells and mice were treated with the stimulant LPS and herbal constituents, and resultant culture supernatant and serum, respectively, were evaluated for interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha by ELISA. All three constituents inhibited LPS-induced IL-6 and/or TNF-alpha production in culture. Inhibition of these two cytokines was observed in mice, but did not display the same patterns of inhibition as cell culture data. The results suggest that all three constituents possessed anti-inflammatory properties, but that cell culture data can only be used to approximate potential effects in animals, and must be confirmed using appropriate animal models. (C) 2003 Elsevier Ltd. All rights reserved.