Characterization of novel squamous cell carcinoma antigen-related molecules in mice
Characterization of novel squamous cell carcinoma antigen-related molecules in mice
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DOI：
10.1016/j.bbrc.2004.09.204
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发表时间：
2004-11-26
影响因子：
3.1
通讯作者：
Izuhara, K
中科院分区：
文献类型：
--
作者：
Sakata, Y;Arima, K;Izuhara, K
鳞状细胞癌抗原1 （SCCA1）和SCCA2是独特的蛇形蛋白，可以抑制半胱氨酸蛋白酶。它们的小鼠同源物SQN-5已被鉴定，其抑制特性已被表征；然而，其生物学作用仍未明确。此外，还没有发现SQN-5的其他小鼠同源物。我们在这篇文章中描述了三种scca相关分子的小鼠成员，包括SQN-5。Serpinb3a （SQN-5）和Serpinb3b（而不是Serpinb3c）具有功能性，由于其活性位点环中两种氨基酸的差异，它们抑制丝氨酸和半胱氨酸蛋白酶的抑制谱不同。Serpinb3a在大多数组织中普遍表达，而Serpinb3b的表达仅限于角质形成细胞。角质形成细胞同时分泌scca相关蛋白Serpinb3a和Serpinb3b。这些结果表明，Serpinb3a和Serpinb3b可能通过抑制不同表达分布和不同抑制谱的内源性或外源性蛋白酶发挥不同的作用。(C) 2004爱思唯尔公司版权所有。
The squamous cell carcinoma antigen 1 (SCCA1) and SCCA2 are unique serpins that can inhibit cysteine proteinases. SQN-5, their mouse ortholog, has already been identified, and its inhibitory property has been characterized; however, its biological role has remained undefined. Furthermore, no other mouse homolog of SQN-5 has been known. We characterize three mouse members of SCCA-related molecules including SQN-5 in this article. Serpinb3a (SQN-5) and Serpinb3b, but not Serpinb3c, were functional, inhibiting both serine and cysteine protemases with different inhibitory profiles due to the difference of two amino acids in their reactive site loops. Serpinb3a was ubiquitously expressed in most tissues, whereas expression of Serpinb3b was limited to keratinocytes. Keratinocytes secreted both SCCA-related proteins, Serpinb3a and Serpinb3b. These results indicate that Serpinb3a and Serpinb3b may play different roles by inhibiting intrinsic or extrinsic protemases with different expression distributions and different inhibitory profiles. (C) 2004 Elsevier Inc. All rights reserved.