“Enzyme Kinetics and Mechanism” by Paul F. Cook and W. W. Cleland
“Enzyme Kinetics and Mechanism” by Paul F. Cook and W. W. Cleland
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Paul F. Cook 和 W. W. Cleland 的“酶动力学和机制”
DOI：
10.1080/14756360701746591
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发表时间：
2008
影响因子：
5.6
通讯作者：
Irene Lee
中科院分区：
文献类型：
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作者：
Irene Lee
本书主要是为研究生设计的教科书，也可能是高级研究员的教学手册，描述了如何利用各种酶动力学技术来阐明酶反应的机制。前言概括了这篇关于阐明酶机制的优秀论文的精髓。本书以教程的形式系统地组织，并遵循研究人员在对酶进行机械表征时所做的实验的逻辑顺序，从动力学技术开始。清晰地介绍了实验设计、数据采集和分析的基本原理，以及编译动力学数据以构建合理的反应机制的实践，以便读者可以轻松掌握并培养解决更复杂和复杂的酶机制所需的技能。第 1 章至第 4 章可归类为介绍性章节。第 1 章介绍了广泛应用于酶动力学领域的 Cleland 命名系统。第 2 章概述了酶学中常见的动力学原理。第 3 章中介绍的材料提供了设计动力学测定的实用信息，第 4 章逻辑上涵盖了数据分析，以及控制第 3 章中获得的实验数据的数学处理的基本原理。令人印象深刻的是，作者花时间为读者提供了必要的数学背景，以指导他们推导速率方程。这些部分对酶学领域的新手和专家都有好处。第 5 章和第 6 章逻辑上建立在前四章提供的背景之上。这两章简要介绍了常见的酶机制，再次使用教程格式来明确描述材料。与前面的章节一样，作者付出了大量的努力来清楚地解释数据采集和分析的“如何”方面。作者没有详尽概述所有可能的酶机制的诊断模式，而是使用具体而清晰的例子系统地解释了推导酶机制和相关速率方程的基本原理。如果可用，将提供与这些具体示例相关的主要参考资料，以便读者可以更详细地探索这些机制。纳入实际考虑因素进一步强调了本书的“教学”方面。抑制研究的覆盖范围，特别是解释抑制的基本原理和类型，是全面的。第 7 章至第 10 章提供了推导酶机制的其他技术。第 7 章对瞬态动力学方法进行了综述，该方法可用于使用第 5 章和第 6 章中介绍的方法进一步定义动力学机制中定义的微观步骤。第 8 章到第 10 章更深入地介绍了利用同位素作为补充工具来解决酶反应的动力学和化学机制。 pH 速率曲线研究的执行以及相关数据的解释和使用以非常适合读者的方式进行教授。提供的示例说明了这些方法，并为读者在整个学习过程中提供了有用的指导。完成本书概述的教程过程后，读者将很好地掌握设计和执行适当动力学实验的原理，最终根据推导出的动力学数据构建合理的酶反应机制。本书中教授的方法将是很好的自学指南，也是酶动力学入门课程的好教材。
This book, designed primarily as a text for graduate students and apossibly a teachingmanual for the senior investigator, describes the utilization of various enzyme kinetic techniques to elucidate the mechanisms of enzymatic reactions. The preface encapsulates the essence of this excellent treatise on elucidating enzyme mechanisms. The book is systematically organized in a tutorial format and follows a logical order of what experiments an investigatorwoulddowhile performing mechanistic characterization of an enzyme, initiating with kinetic techniques. The underlying principles for experimental design, data acquisition and analyses, as well as the practice of compiling the kinetic data to construct a plausible reaction mechanism, are clearly presented so that the reader can readily follow and develop the necessary skills to tacklemore complex and sophisticated enzyme mechanisms. Chapters 1 to 4 can be categorized as the introductory chapters. The Cleland nomenclature system, widely used in the field of enzyme kinetics, is introduced in Chapter 1. Chapter 2 provides an overview of the commonly encountered kinetic principles in enzymology. The material presented in Chapter 3 provides practical information for designing kinetic assays, which is logically followed by Chapter 4 which covers data analysis, and the underlying principles that govern the mathematical treatment of the experimental data obtained in Chapter 3. It is quite impressive that the authors have taken the time to provide the reader with the necessary mathematical background to guide them through the derivation of rate equations. These sections are beneficial for both novices and experts in field of enzymology. Chapters 5 and 6 logically build upon the background provided in the first four chapters. These two chapters provide a concise account of the commonly encountered enzyme mechanisms, again using a tutorial format to explicitly describe the material. As in earlier chapters, the authors have placed a substantial amount of effort to clearly explain the “how to” aspects of data acquisition and analysis. Rather than providing an exhaustive overview of diagnostic patterns of all possible enzyme mechanisms, the authors systematically explain the rationale for deducing the enzyme mechanism and the associated rate equations using specific and clear examples. When available, primary references associated with these specific examples are provided so that the reader may explore these mechanisms in further detail. The inclusion of practical considerations further underscores the “teaching” aspect of this book. Coverage of inhibition studies, especially on explaining the underlying principles and the types of inhibition, is comprehensive. Chapters 7 to 10 provide additional techniques for deducing enzyme mechanisms. Chapter 7 provides a survey of transient kinetic methodologies that can be used to further define the microscopic steps defined in a kinetic mechanism using the approaches presented in Chapters 5 and 6. Chapters 8 through 10 offer more in-depth coverage of the utilization of isotopes as complementary tools to solve the kinetic and chemical mechanisms of enzymatic reactions. The execution of pH rate-profile studies as well as the interpretation and usage of the associated data are taught in a very reader friendly manner. Examples are provided that illustrate the approaches and provide helpful guides for the readers through the entire learning process. After completing the tutorial process outlined in this book, the reader will have obtained an excellent grasp on the rational for designing and executing proper kinetic experiments which will culminate in the construction of a plausible enzymatic reaction mechanism from the deduced kinetic data. The approaches taught in this text will serve a good self-tutorial guide as well as a good text for an introductory course in enzyme kinetics.